%0 Journal Article
%T IL-18基因联合二萜生物碱对舌鳞癌细胞的增殖抑制与促凋亡作用<br>Effects of IL-18 gene combined with diterpenoid alkaloids in inducing proliferation and promoting apoptosis of tongue squamous carcinoma cells
%A 胡雅琳
%A 侯美玲
%A 郑文娇
%A 张泽南
%A 何韶衡
%A 李志刚
%J 西安交通大学学报(医学版)
%D 2017
%R 10.7652/jdyxb201702012
%X 摘要：目的 探讨人IL-18基因联合二萜生物碱对舌鳞癌细胞Tscca的增殖抑制与促凋亡作用。方法 构建pEGFPN3-IL-18质粒，转染舌鳞癌细胞Tscca。荧光显微镜下观察质粒的转染效率；MTT检测pEGFPN3-IL-18联合二萜生物碱对舌鳞癌细胞Tscca生长的影响；流式细胞术检测细胞凋亡情况；蛋白质免疫印迹法检测细胞信号调节激酶Akt/p-Akt的蛋白水平。结果 转染pEGFPN3-IL-18的舌鳞癌细胞Tscca较未转染细胞凋亡数增加，差异有统计学意义(P<0.05)；0.2、0.4、0.6mg/mL二萜生物碱培养舌鳞癌细胞，随浓度增加，细胞凋亡数增多，差异有统计学意义(P<0.05)；IL-18与二萜生物碱联合对Tscca细胞作用48h后，较单独使用二萜生物碱的增殖抑制作用明显，且呈浓度依赖性，差异有统计学意义(P<0.05)；IL-18联合二萜生物碱还可剂量依赖性地降低 p-Akt的蛋白水平。结论 pEGFPN3-IL-18联合二萜生物碱对舌鳞癌细胞Tscca有协同抑制作用。<br>ABSTRACT: Objective To investigate the effects of human IL-18 gene combined with diterpenoid alkaloids in inhibiting the proliferation and inducing the apoptosis of tongue squamous carcinoma cells Tscca. Methods We constructed recombinant plasmid pEGFPN3-IL-18 and tranfected it into tongue squamous carcinoma cells Tscca. The transduction efficiency of the target cells was detected by fluorescent microscopy, cytotoxic effect of IL-18 gene with diterpenoid alkaloids on Tscca was detected by MTT assay, and apoptosis was detected by flow cytometry. Western blot was employed to examine the expression level of cellular signal-regulated kinase Akt/p-Akt. Results The tongue squamous cells Tscca which transfected pEGFPN3-IL-18 had significantly increased apoptosis compared with non-transfected cells (P<0.05). Tongue carcinoma squamous cells cultured with diterpenoid alkaloids at the concentrations of 0.2, 0.4 and 0.6mg/mL had significantly increased apoptosis in a dose-dependent manner (P<0.05). Human IL-18 gene combined with diterpenoid alkaloids for 48 hours inhibited significantly Tscca in a concentration-dependent manner compared with diterpenoid alkaloids alone (P<0.05). The two in combination could also decrease the protein level of p-Akt dose-dependently. Conclusion The combination of pEGFPN3-IL-18 and diterpenoid alkaloids has a synergistic effect in inhibiting the growth of tongue squamous carcinoma cells Tscca
%K 白细胞介素18(IL-18)
%K 二萜生物碱
%K 联合用药
%K 舌鳞状细胞癌
%K 增殖抑制
%K 凋亡&lt
%K br&gt
%K interleukin-18 (IL-18)
%K diterpenoid alkaloid
%K combination therapy
%K squamous cell carcinoma
%K inhibition of proliferation
%K apoptosis
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201702012