%0 Journal Article
%T 复方续断三七提取物对家兔骨折愈合的促进作用及其机制<br>Effect and mechanism of the compound of radix dipsaci and pseudo-ginseng extract on bone fracture in rabbits
%A 宁
%A 涛
%A 胡
%A 勇
%J 西安交通大学学报(医学版)
%D 2016
%R 10.7652/jdyxb201606023
%X 摘要：目的 观察复方续断三七提取物(compound of radix dipsaci and pseudo-ginseng extract, CRPE)对家兔骨折愈合的促进作用，并探讨其机制。方法 采用家兔胫骨骨折模型，分成假手术组、模型组及CRPE 0.6g/kg、CRPE 1.2g/kg和CRPE 2.4g/kg组。观察CRPE对家兔骨骼愈合过程中骨痂形成时间、骨折愈合时间、血清中转化生长因子1(TGF-β1)、Ⅰ型胶原、Ⅱ型胶原、碱性磷酸酶、钙、磷、锌、铜含量的影响。采用Western blot和RT-PCR观察CRPE对家兔胫骨骨折组织中P-Smad2、P-Smad3、Smad7、PAI-1蛋白及mRNA的表达影响。结果 CRPE(1.2g/kg)组和CRPE(2.4g/kg)组能缩短骨痂形成时间和骨折愈合时间，显著升高骨折愈合过程中所需的TGF-β1、Ⅰ型胶原、Ⅱ型胶原、碱性磷酸酶、钙、锌、铜的含量；升高P-Smad2、P-Smad3和PAI-1的蛋白及mRNA的表达，降低Smad7蛋白及mRNA的表达(P<0.05)。结论 CRPE对家兔骨折预后有显著的促进作用，其机制可能与TGF-β1/Smad信号通路有关。<br>ABSTRACT: Objective To evaluate the effect and mechanism of the compound of radix dipsaci and pseudo-ginseng extract (CRPE) on bone fracture in rabbits. Methods The model of rabbit tibia fracture was used to detect the effects of CRPE on callus formation time and healing time. TGF-β1, collagen type Ⅰ, collagen type Ⅱ, alkaline phosphatase, Ca, P, Zn, Cu in rabbit serum were also measured. The protein and mRNA of P-Smad2, P-Smad3, Smad7 and PAI-1 were detected by RT-PCR and Western blot to evaluate the protective effect of CRPE. Results CRPE (1.2g/kg and 2.4g/kg) could significantly shorten callus formation time and healing time; and increase TGF-β1, collagen type Ⅰ, collagen type Ⅱ, alkaline phosphatase, Ca, P, Zn, Cu levels in rabbit serum. In addition, CRPE(1.2g/kg and 2.4g/kg) could increase P-Smad2, P-Smad3 and PAI-1 expressions and reduce the expression of Smad7 (P<0.05). Conclusion CRPE can improve the prognosis of bone fracture in rabbits and its mechanism might be related to TGF-β/Smad pathway
%K 复方续断三七提取物
%K 骨折
%K 转化生长因子1(TGF-β1)
%K Smad
%K PAI-1&lt
%K br&gt
%K compound of radix dipsaci and pseudo-ginseng extract
%K bone fracture
%K TGF-β1
%K Smad
%K PAI-1
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201606023