%0 Journal Article
%T 重组人松弛素-2对肝星状细胞增殖、Ⅰ型胶原合成及TGF-β1与CTGF mRNA表达的影响<br>Effects of recombinant human relaxin-2 on hepatic stellate cell proliferation, synthesis of Collagen Ⅰ, and mRNA expressions of TGF-β1 and CTGF in vitro
%A 陈
%A 振
%A 许君望
%A 严
%A 肃
%A 陈
%A 珂
%A 范修德
%J 西安交通大学学报(医学版)
%D 2017
%R 10.7652/jdyxb201701009
%X 摘要：目的 在细胞水平研究松弛素的抗肝纤维化作用，并初步探讨其分子机制，为肝纤维化的治疗提供实验依据。方法 体外培养大鼠肝星状细胞(HSC-T6)，分别用20、50、100ng/mL重组人松弛素-2(recombinant human relaxin-2, RLX-2)干预，采用噻唑蓝(MTT)比色实验检测RLX-2对HSC-T6增殖的影响，双抗体夹心酶联免疫吸附法(ELISA)检测对照组及RLX-2作用48h后的细胞培养基上清液Ⅰ型胶原水平，利用逆转录酶-聚合酶链反应(RT-PCR)技术检测药物干预48h的结缔组织生长因子(CTGF)、转化生长因子-β1(TGF-β1)mRNA表达情况。结果 RLX-2对HSC的增殖具有抑制作用，可降低HSCⅠ型胶原水平，RLX-2抑制HSC的CTGF mRNA表达，对HSC的TGF-β1 mRNA表达无明显影响。结论 RLX-2可能通过抑制体外培养大鼠HSC细胞的增殖、Ⅰ型胶原及CTGF mRNA的表达而发挥抗大鼠肝纤维化作用。<br>ABSTRACT: Objective To validate that relaxin can resist hepatic fibrosis at the cellular level and explore its molecular mechanism in order to provide experimental basis for the treatment of liver cirrhosis. Methods Cultured HSC-T6s were treated with different concentrations (20, 50 and 100ng/mL) of recombinant human relaxin-2 (RLX-2). The proliferation of HSC-T6 was measured by MTT colorimetric assay. The content of type Ⅰ collagen in the cell culture supernatant of each group was detected by ELISA at 48h of drug intervention; RT-PCR was used to detect the mRNA expressions of CTGF and TGF-β1 in HSC-T6 at 48h of drug intervention. Results RLX-2 inhibited the proliferation of HSC and reduced type Ⅰ collagen content of HSC cells. It also inhibited the CTGF mRNA expression of HSC, but did not have a significant effect on the expression of TGF-β1 mRNA. Conclusion In the experiment of culturing HSC-T6 in vitro, RLX-2 may play a role in rat liver fibrosis by inhibiting cell proliferation and type Ⅰ collagen and CTGF mRNA expressions
%K 肝纤维化
%K 肝星状细胞
%K 结缔组织生长因子(CTGF)
%K 转化生长因子(TGF)
%K 松弛素&lt
%K br&gt
%K hepatic fibrosis
%K hepatic stellate cell
%K connective tissue growth factor(CTGF)
%K transforming growth factor(TGF)
%K relaxin
%U http://yxxb.xjtu.edu.cn//oa/darticle.aspx?type=view&id=201701009