%0 Journal Article
%T 参与烟粉虱免疫反应的clip丝氨酸蛋白酶基因分析<br>Analysis on clip serine protease genes involved in innate immunity of Bemisia tabaci (Hemiptera: Aleyrodidae)
%A 于洁
%A 王登杰
%A 张林雅
%A 雷仲仁
%A 王海鸿
%A Yu Jie
%A Wang Dengjie
%A Zhang Linya
%A Lei Zhongren
%A Wang Haihong
%J 植物保护学报
%D 2016
%R 10.13802/j.cnki.zwbhxb.2016.01.008
%X 为了解参与烟粉虱免疫反应的clip丝氨酸蛋白酶(clip-domain serine proteases in Bemisia tabaci,BtCLIPs)基因,对已鉴定的8个BtCLIPs基因编码的蛋白进行序列分析,与冈比亚按蚊、家蚕、黑腹果蝇、烟草天蛾4种模式昆虫的同源蛋白进行系统进化分析,并通过qRT-PCR检测球孢白僵菌侵染烟粉虱4龄若虫后BtCLIPs的时间表达模式.结果显示,8个BtCLIPs基因均为含有CLIPs结构域的丝氨酸蛋白酶基因;BtCLIP19、BtCLIP20和BtCLIP21独自聚在进化树的一支上;其它BtCLIPs均与4种模式昆虫中的1种或多种同源蛋白聚在一起;与对照相比,8个BtCLIPs基因受球孢白僵菌侵染后在不同时间的相对表达量均上调,但诱导程度不同,48 h的相对表达量达最高,其中BtCLIP19上调表达最明显,为对照组的71倍.研究表明,8个BtCLIPs可能参与烟粉虱对真菌侵染的免疫反应,并成为烟粉虱生物防治的新靶标.<br>To study clip-domain serine proteases in tobacco whitefly Bemisia tabaci, eight CLIP serine proteases identified in B. tabaci were analyzed by protein sequence alignment and phylogenetic analysis with homologous CLIPs in Anopheles gambiae, Bombyx mori, Drosophila melanogaster and Manduca sexta. The 4th instar B. tabaci nymphs were infected by Beauveria bassiana and the expression of clips serine protease genes was subsequently analyzed by qRT-PCR. The results showed that the eight BtCLIPs genes contained CLIP domain; BtCLIP19, BtCLIP20 and BtCLIP21 formed a specific clade. The other BtCLIPs were evolutionarily close to one or more homologous serine proteases in the four model insects. The transcriptions of eight BtCLIPs genes in B. tabaci infected by B. bassiana were up-regulated and peaked at 48 h. The transcription of BtCLIP19 was the highest among all BtCLIPs genes and increased 71 folds compared to the control. The eight BtCLIPs genes probably participated in the innate immune responses to the fungus infection, and could be a new target for controlling B. tabaci.
%K 烟粉虱 丝氨酸蛋白酶 clip结构域 免疫反应&lt
%K br&gt
%K Bemisia tabaci serine protease clip domain immunological reaction
%U http://www.zwbhxb.cn/ch/reader/view_abstract.aspx?file_no=20160108&flag=1