%0 Journal Article
%T 高产脂肪酶菌株的筛选鉴定<br>Screening of high lipase-producing strains
%A 谢清
%A 王瑶
%A 陈尚武
%A 李平兰
%J 中国农业大学学报（自然科学中文版)
%D 2018
%X 通过变色圈法和透明圈法从泥土中筛选出脂肪酶产生菌，并根据菌株的菌落形态、革兰氏染色和16S rDNA序列分析确定筛选得到菌株的种属。采用罗丹明B （Rhodamine B）平板初筛出51株脂肪酶产生菌，以荧光圈直径或透明圈直径与菌落直径的比值（HC）为指标，选取HC>1.60的9株脂肪酶产生菌，采用橄榄油乳化液平板进行复筛，对HC进行测量比较筛选出1株高产脂肪酶的菌株L-17，其HC为2.27。利用透明圈法对菌株L-17的粗酶液的酶活力进行测定，HC为3.07，表明菌株L-17具有较高的酶活力。菌株L-17经初步鉴定为洋葱伯克霍尔德菌（Burkholderia cepacia），为一株高产脂肪酶的菌株，具有潜在的研究和开发价值。<br>Lipase-producing strains isolated from soil were screened by the color-changing circle method and transparent circle method.These strains were identified according to their morphology,Gram staining and 16S rDNA sequence analysis.A total of 51 high lipase-producing strains were isolated on Rhodamine B screening plates by using HC index.Compared the HC of the strains screened by olive oil screening plates,L-17,the strain of highest HC 2.27,was identified as target strain.The enzyme activity of L-17 was determined by transparent circle method,and its HC was 3.07 which proved the high enzyme activity of L-17.The L-17 strain was identified as Burkholderia cepacia with high lipase yield,which had potential research and development value.
%K 脂肪酶 筛选 鉴定 洋葱伯克霍尔德菌&lt
%K br&gt
%K lipase screening identification Burkholderia cepacia
%U http://zgnydxxb.ijournals.cn/zgnydxxb/ch/reader/view_abstract.aspx?file_no=20180510&flag=1