%0 Journal Article
%T 动态高压微射流对乳蛋白抗原性的影响<br>Effects of dynamic high-pressure microfluidization on antigenicity of milk protein concentrate
%A 许倩
%A 李大鹏
%A 黄和平
%A 石径
%A 罗永康
%J 中国农业大学学报（自然科学中文版)
%D 2015
%X 为评价浓缩乳蛋白(Milk protein concentrate)溶液经动态高压微射流(Dynamic high-pressure microfluidization,DHPM)及DHPM结合加热(65℃ 30 min和95℃ 30 min)处理过程中α-乳白蛋白、β-乳球蛋白、α-酪蛋白和β-酪蛋白抗原性的变化,采用间接竞争ELISA法测定不同压力条件下MPC中各蛋白抗原性的变化。结果表明:1)4种蛋白抗原性对DHPM及DHPM结合加热的反应各不相同。2)DHPM+65℃/95℃处理后,蛋白质α-乳白蛋白、α-酪蛋白和β-酪蛋白抗原性均明显低于DHPM处理样和水化对照样。因此,DHPM及DHPM结合加热处理可降低MPC中酪蛋白的抗原性,增加α-乳白蛋白的抗原性,但对β-乳球蛋白效果不明显。<br>Milk protein concentrates were treated by dynamic high-pressure microfluidization (DHPM) and DHPM combined with heat treatment (65℃,95℃ for 30 min).Indirect competitive ELISA method was used to determine the antigenicity of α-lactalbumin, β-lactoglobulin, α-casein and β-casein in MPC under different conditions.The results showed that the reactions of four antigenic proteins to DHPM and DHPM combined with heat treatment were not identical.The antigenicity of α-lactalbumin、 α-casein and β-casein were significantly lower than DHPM treatment samples and hydrating sample.
%K 动态高压微射流 加热 浓缩乳蛋白 抗原性&lt
%K br&gt
%K dynamic high-pressure microfluidization heat treatment milk protein concentrates antigenicity
%U http://zgnydxxb.ijournals.cn/zgnydxxb/ch/reader/view_abstract.aspx?file_no=20150530&flag=1