%0 Journal Article
%T 一株高环多环芳烃降解嗜盐菌Thalassospira sp.的分离及降解特性<br>Isolation and degradation characteristics of a HMW-PAHs degrading halophilic strain
%A 王慧()
%A 周海燕
%A 黄勇
%A 方婷婷
%J 清华大学学报（自然科学版）
%D 2015
%X 为获得盐环境中高环多环芳烃(HMW-PAHs)污染修复的高效降解菌株,该文以芘为唯一碳源和能源,利用5%盐度矿物培养基(MSM)从石油污染土壤中富集分离出一株能降解菲、荧蒽、芘、苯并蒽的嗜盐菌,经形态学观察和16SrRNA序列鉴定为海旋菌(Thalassospira), 命名为Thalassospira sp.strain TSL5-2。在5%盐度下, 25d内, TSL5-2对菲、芘、荧蒽(初始质量浓度均为20mg/L)、 苯并蒽(初始质量浓度为8mg/L)的降解率分别为100%、 53.3%、 60%、 18.1%,但不能降解苯并芘。TSL5-2耐盐范围为0.5%~19.5%,最适盐度为5%。外加酵母粉和蛋白胨(5mg/L)对菲的降解都有一定的促进作用,后者效果更为明显, 5d内菲的降解率分别为60.9%和82.1%,高于MSM对照组(46.1%)。酵母粉对TSL5-2降解芘和苯并芘的影响效果都不显著。蛋白胨对芘降解有抑制作用,但对苯并芘(5mg/L)降解有促进效果, 25d的芘和苯并芘的降解率分别为17.5%和38.2%。<br>Abstract： A halophilic cell strain was isolated that efficiently degrades high molecular weight-polycyclic aromatic hydrocarbon (HMW-PAHs) contamination in saline environments. This strain, which can degrade phenanthrene, fluoranthene, pyrene and benzanthracene, was isolated from oil-polluted soil with pyrene as the sole carbon and energy source in a mineral salt medium (MSM) of 5% salinity. The strain was identified as Thalassospira based on morphological observations and an 16SrRNA sequence and named Thalassospira sp. strain TSL5-2. After 25 days' cultivation in 5% salinity culture solution, strain TSL5-2 degraded phenanthrene by 100%, pyrene by 53.5%, fluoranthene by 60% (initial concentration: 20mg/L) and benzanthracene by 18.1% (initial concentration: 8mg/L), but could not degrade benzopyrene. TSL5-2 had a wide salinity tolerance range (0.5%-19.5%), with an optimal salinity of 5%. Adding yeast powder and peptone (5mg/L) to the MSM both improved the degradation of phenanthrene by TSL5-2, with degradation rates of 60.1% with yeast and 82.1% with peptone at day 5, both higher than the control group (46.1%). Yeast powder did not effectively improve the degradation of pyrene and benzopyrene. Peptone inhibited the degradation of pyrene, while accelerating benzopyrene degradation, with a pyrene degradation rate of 17.5% and a benzopyrene (5mg/L) degradation rate of 38.2% at 25 days.
%K 芘
%K 嗜盐菌分离
%K Thalassospira菌
%K 降解
%K &lt
%K br&gt
%K School of Environment
%K Tsinghua University
%K Beijing 100084
%K China
%U http://jst.tsinghuajournals.com/CN/Y2015/V55/I1/87#ReferenceTab