%0 Journal Article
%T 微流体流式细胞仪的关键技术<br>Key techniques in microfluidic flow cytometers
%A 赵精晶
%A 尤政
%J 清华大学学报（自然科学版）
%D 2018
%R 10.16511/j.cnki.qhdxxb.2018.26.036
%X 流式细胞仪是用于高通量细胞分析和分选的高端生命科学仪器，被广泛应用于科学研究和临床诊断。最新的一个发展方向是以微流体芯片为核心的高集成度、微体积、全封闭、零交叉污染的微流体流式细胞仪。该文针对微流体流式细胞仪的三维（3-D）样本聚焦、光斑整形和片上分选等3项关键技术开展了研究。3-D流体动力聚焦微流体芯片能够在每秒数米的高流速下将样本流聚焦在流道中心，聚焦后的样本流边长仅为10 μm量级。经设计的二元光学器件可将激光光斑整形为矩形准平顶光斑，比传统流式所使用的椭圆形Gauss光斑，矩形平顶光斑具有更均一的能量分布。采用电火花空化微气泡，利用空泡膨胀产生的射流实现高精度的单细胞片上分选。将整合了上述技术的微流体流式细胞仪应用于标准荧光微球的分析测试，综合性能达到或接近于现有的大型传统流式细胞仪。<br>Abstract：Flow cytometers are biomedical instruments for high throughput analyses and sorting of single cells which are widely used in both biomedical research and clinical diagnoses. Advances in microfluidics have led to highly-integrated, compact, fully-enclosed, and no cross-contamination microfluidic flow cytometers. This study focuses on three key techniques with 3-D focusing of the sample flow, laser beam shaping, and on-chip sorting. 3-D focusing microfluidic chips confine the sample flow down to 10 micrometers at a velocity of several meters per second. Specially-designed binary optical elements (BOEs) generate micrometer-scale rectangular quasi-flat spots for exciting fluorescence that replace conventional elliptical Gaussian spots. These give an on-chip sorting mechanism based on the jet-flow by expanding spark-induced cavitation microbubbles. A microfluidic test system is developed by combining these three techniques with performance that is close to that of two commercial instruments.
%K 流式细胞仪
%K 微流体
%K 细胞分选
%K 二元光学器件
%K 荧光检测
%K 流式细胞术
%K &lt
%K br&gt
%K flow cytometer
%K microfluidics
%K cell sorting
%K binary optical element
%K fluorescence detection
%K flow cytometry
%U http://jst.tsinghuajournals.com/CN/Y2018/V58/I11/953