%0 Journal Article
%T 八周耐力训练介导Nrf2对骨骼肌谷胱甘肽抗氧化系统的影响<br>Effects of Eight-Week Endurance Training on Glutathione Antioxidant System of Skeletal Muscles by Nrf2 Signal Pathway
%A 毛雅芸
%A 刘雨佳
%A 张缨
%J 北京体育大学学报
%D 2017
%R 10.19582/j.cnki.11-3785/g8.2017.02.008
%X 摘要：目的：探讨八周耐力训练对骨骼肌谷胱甘肽抗氧化系统的影响，以及Nrf2基因缺失的作用。方法：C57BL/6J野生型小鼠、Nrf2基因敲除小鼠各20只，随机分为安静对照组和耐力训练组，每组10只。耐力训练组采取坡度为0°，速度为12 m/min的跑台训练，1 h/d，5 d/周，共8周。完成训练48 h后取小鼠腿部骨骼肌，Western blot测定骨骼肌Nrf2、GSH-Px、GR、GCLc和GCLm蛋白表达，酶还原法测定骨骼肌GSH、GSSG含量。结果：1）Nrf2敲除鼠与野生鼠相比，骨骼肌内GSH-Px（P＜0.05）、GR（P＜0.05）、GCLc（P＜0.01）蛋白表达，GSH（P＜0.01）、GSSG（P＜0.05）含量和GSH/GSSG比值（P＜0.05）非常显著性或显著性下降，而GCLm蛋白表达无明显差异。2）耐力训练组与安静对照组相比，野生训练鼠骨骼肌Nrf2、GSH-Px、GCLc、GCLm以及GSH/GSSG比值均呈上升趋势，GSSG含量减少；Nrf2敲除训练鼠的谷胱甘肽相关酶蛋白表达以及GSH/GSSG比值则表现下降趋势，GSSG含量上升，但并无显著性。结论：Nrf2的缺失导致耐力训练不但没有提高机体抗氧化能力，反而加剧机体抗氧化水平的下降。<br>Abstract: Objective: The study aimed at investigating the effect of eight-week endurance training on glutathione antioxidant system and the role of nuclear factor erythroid-2 related 2(Nrf2). Methods: Twenty two-month-old C57BL/6J wild-type(WT) mice and twenty Nrf2 knock-out(KO) mice were divided into control and endurance exercise groups (n=10). Mice in exercise groups underwent 60 min/day treadmill running at speed of 12 m/min for 8 weeks. Skeletal muscle was collected 48 hours after training. The protein expressions of Nrf2, GSH-Px, GR, GCLc and GCLm in skeletal muscle were analyzed by Western blot. The GSH and GSSG levels were tested by Enzyme reduction method. Results: 1) Compared with WT mice, the expressions of GSH, GSSG, GSH/GSSG, GSH-Px, GR and GCLc of skeletal muscle in Nrf2 knock-out mice were significantly decreased while GCLm had no difference. 2) Compared with control group, the expressions of Nrf2, GSH-Px, GCLc and GCLm and GSH/GSSG value of WT mice in exercise group increased while GSSG decreased, the expressions of glutathione related enzymes and GSH/GSSG value of Nrf2 knock-out mice in exercise group decreased while GSSG increased, but no difference. Conclusions: Conclusion: The genetic depletion of Nrf2 doesn’t improve antioxidant capacity, and it decreases antioxidant level
%K Nrf2 knock-out endurance training skeletal muscle glutathione antioxidase
%U http://xuebao.bsu.edu.cn/ch/reader/view_abstract.aspx?file_no=20170208&flag=1