%0 Journal Article
%T 低氧暴露不同时间对小鼠骨骼肌apelin/APJ表达的影响<br>Effects of Different Duration of Hypoxia on Apelin/APJ Expression in Skeletal Muscle of Mice
%A 姬卫秀
%A 张缨
%J 北京体育大学学报
%D 2018
%R 10.19582/j.cnki.11-3785/g8.2018.12.010
%X 摘要：低氧可能对机体代谢异常有着积极的调节作用。Apelin是G蛋白偶联受体APJ的内源性配体，参与骨骼肌能量代谢的调节。目的：采用动物实验，观察低氧暴露不同时间对小鼠骨骼肌apelin表达的影响；通过细胞实验进一步探讨HIF-1α是否参与了低氧对小鼠骨骼肌apelin表达的调节。方法：动物实验采用C57BL/6J雄性小鼠40只，随机分为对照组（0h）和不同时间低氧暴露组（6h、24h、48h），每组10只，共4组。低氧组氧浓度约为11.2%，低氧暴露结束后即刻取材。细胞实验采用C2C12细胞，分为常氧对照组（N）、低氧暴露组（H）、低氧对照组（H+siControl）和低氧HIF-1α沉默组（H+siHIF-1α），共4组。转染48 h沉默后，在低氧浓度为5%的低氧中培养72 h，收集细胞。Western blot 测定骨骼肌组织和细胞apelin、APJ、HIF-1α的蛋白表达。RT-PCR测定骨骼肌中apelin、APJ、HIF-1α mRNA含量。结果：1）与0h组相比，6h、24h和48h组小鼠骨骼肌apelin/APJ mRNA和蛋白及HIF-1α mRNA表达均增加。与6h组相比，48h组小鼠骨骼肌apelin mRNA及HIF-1α mRNA和蛋白表达量增加。与24h组相比，48h组小鼠骨骼肌apelin和HIF-1α mRNA表达量显著增加。2）与N组相比，H组细胞apelin、APJ、HIF-1α蛋白表达增加。与H组和H+siControl组相比，H+siHIF-1α组细胞apelin、APJ、HIF-1α蛋白表达降低。结论：模拟常压低氧暴露（11.2%氧浓度）6 h可显著增加小鼠骨骼肌apelin/APJ的表达，并且其表达水平呈时间依赖性；HIF-1α参与了低氧对骨骼肌apelin/表达的调节作用。揭示了低氧与骨骼肌apelin表达的关系，为低氧暴露促进健康提供理论依据。<br>Abstract: Hypoxia may have a positive role in the regulation of metabolic abnormalities. Apelin is the endogenous ligand for G protein-coupled receptor APJ, it can adjust the energy metabolism in skeletal muscle. Objective: To investigate the effect of different duration of hypoxia on the expression of apelin/APJ in skeletal muscle of mice by animal experiment; to detect whether HIF-1α is involved in the regulation of apelin expression in mice skeletal muscle by hypoxia through cell experiment. Methods: In animal experiment, 40 male C57BL/6j mice were divided into control group (0 h) and different duration hypoxia groups (6 h, 24 h, 48 h) randomly. There were 10 mice in each group and 4 groups in total. The oxygen concentration in hypoxia group was about 11.2%. The mice were sacrificed instantly after hypoxic exposure. In cell experiment, C2C12 cells were allocated into four groups: normoxia group (N), hypoxic exposure group (H), hypoxic exposure control group (H+siControl) and hypoxic exposure with HIF-1α silence group (H+siHIF-1α). After 48 h transfection, the cells were cultured for 72 h at 5% oxygen concentration and then were collected. The protein expressions of apelin, APJ and HIF-1α in skeletal muscle tissue and cells were tested by Western blot; and the mRNA expressions of apelin, APJ, HIF-1α mRNA were measured by RT-PCR. Results: 1) Compared with 0 h group, the expressions of apelin/APJ and HIF-1α mRNA in skeletal muscle in 6 h, 24 h and 48 h groups increased. Compared with the 6 h group, the expressions of apelin mRNA and HIF-1α in 48 h group increased significantly. Compared with 24 h group, the expression of apelin and HIF-1α mRNA in 48 h group increased significantly. 2) Compared with N group, the expression of apelin, APJ, HIF-1α
%K different duration hypoxia skeletal muscle apelin APJ health
%U http://xuebao.bsu.edu.cn/ch/reader/view_abstract.aspx?file_no=20181210&flag=1