%0 Journal Article %T SarcKATP介导运动预处理对I/R心脏的保护作用<br>Protective Effect of Exercise Preconditioning Mediated by SarcKATP on I/R Heart %A 杨永钟 %A 许思毛 %A 王春亮 %J 北京体育大学学报 %D 2017 %R 10.19582/j.cnki.11-3785/g8.2017.03.009 %X 摘要：目的：探讨细胞膜ATP 敏感性钾通道（sarcKATP）在较大强度运动预处理保护缺血再灌注（I/R）心脏中的作用及其与 Kir6.2 亚基蛋白表达变化的关系。方法：108只成年雄性 SD 大鼠，随机分为安慰剂后假手术对照组（PS）、抑制剂后假手术对照组（DS），运动与安慰剂后假手术对照组（EPS）、运动与抑制剂后假手术对照组（EDS），安慰剂后I/R组（PI）、抑制剂后I/R组（DI），运动与安慰剂后I/R组（EPI）、运动与抑制剂后I/R组（EDI）。除PS、ES、EPS、EDS组进行假手术外，其余4组均建立在体I/R（缺血30 min、再灌注60 min）心脏模型；且术前分别采用对应方式进行干预。观察心电图及左室内压变化，每组随机取8只实验成功者进入后续实验，检测血液cTnI、CK-MB浓度及心肌sarcKATP中Kir6.2亚基蛋白表达水平。结果：与缺血前相比，缺血30 min时各I/R组心电图J点及T波幅度显著增加、Q-T间期显著延长（P＜0.05），再灌注60 min时的J点及T波值显著回落（P＜0.05）。与 PS 组相比，各运动组术前LVSP与±dp/dtmax 显著升高、LVEDP 显著下降（P＜0.05）。与缺血前相比，缺血30 min时各 I/R组 LVSP与 ±dp/dtmax 显著下降、LVEDP 显著升高（P＜0.05），而再灌注60 min 时的异常变化较缺血30 min 时更明显（P＜0.05）；与PS组相比，各I/R组术后血液 cTnI、CK-MB浓度显著升高（P＜0.05）。对I/R引起的上述心电图及左室内压参数、血液指标等异常程度进行组间比较，DI组大于PI组、PI组大于EPI组、EDI组大于 EPI 组（P＜0.05）。此外，运动引起心肌sarcKATP中Kir6.2蛋白表达水平增加。结论：运动锻炼提高了心肌功能，而SarcKATP介导了运动预处理对I/R心脏的保护作用，并可能是通过诱导其亚基Kir6.2的蛋白表达即增加孔道量来实现。<br>Abstract: Objective: The purposes of this study were to explore the role of Sarcolemmal ATP-Sensitive Potassium Channels (sarcKATP) in protective effect of vigorous intensity exercise precondition against ischemia reperfusion damage of heart, and to detect its correlation with Kir6.2. Methods: One hundred and eight male adult SD rats were randomly divided into sham-operation after placebo control group (PS), sham-operation after inhibitor control group (DS), sham-operation after exercise and placebo control group (EPS), sham-operation after exercise and inhibitor control group (EDS), ischemia reperfusion (I/R) after placebo group (PI), I/R after inhibitor group(DI), I/R after exercise and placebo group (EPI), I/R after exercise and inhibitor group (EDI). Group PS, ES, EPS, EDS were sham operated, group PI, DI, EPI, EDI were build I/R (30 min ischemia, 60 min reperfusion) heart model, and each group was carried on the corresponding intervention. Rats’ ECG and left intraventricular pressure (LVSP, LVEDP) were observed; 8 rat of each group were selected and finished the following experiment, their serum cardiac troponin-IcTnI, creatine kinase rsoenzyme (CK-MB) and Kir6.2 subunit of sarcKATP were tested. Results: J point, T wave value and Q-T segment of ECG significantly increased after 30 min myocardial ischemia in PI, DI, EPI, EDI groups (P < 0.05), and J point and T wave were significantly recovered after 60 min reperfusion (P < 0.05). Compared with group PS, LVSP ± dp/dtmax significantly increased before operation in exercise groups, and LVEDP significantly decreased (P < 0.05). After 30 min ischemia, -LVSP and ±dp/dtmax of I/R groups significantly decreased while LVEDP increased (P < 0.05), and %K 运动预处理 心肌缺血再灌注损伤 细胞膜ATP敏感性钾通道 心电图 心功能< %K br> %K Keywords：exercise preconditioning myocardial ischemia reperfusion injury sarcolemmal ATP-sensitive potassium channel ECG cardiac function %U http://xuebao.bsu.edu.cn/ch/reader/view_abstract.aspx?file_no=20170309&flag=1