%0 Journal Article
%T 武隆猪腰枣的组织培养研究<br>Tissue Culture of Jujube (<i>Zizyphus jujuba</i>) Mill. cv. Wulongzhuyaozao
%A 夏清清
%A 刘蓝筠
%A 孙海艳
%A 何桥
%A 李晓林
%A 梁国鲁
%A 朱恒星
%A 郭启高&lt
%A br&gt
%A XIA Qing-qing
%A LIU Lan-jun
%A SUN Hai-yan
%A HE Qiao
%A LI Xiao-lin
%A LIANG Guo-lu
%A ZHU Heng-xing
%A GUO Qi-gao
%J 西南大学学报(自然科学版)
%D 2017
%R 10.13718/j.cnki.xdzk.2017.05.006
%X 以武隆猪腰枣为试材，以茎尖、带芽茎段、节间和叶片为外植体，开展了组织培养研究.结果表明，外植体以带芽茎段最好；适宜的初代培养基以1/2 MS+1 mg/L 6-BA+0.2 mg/L NAA，接种的带芽茎段萌芽快，其愈伤组织的不定芽再生率达53.3%；最佳不定芽继代培养基为MS+1 mg/L 6-BA+0.2 mg/L IBA，增殖系数达3.77；不定芽在1/2 MS+0.3 mg/L IBA和1/2 MS+0.3 mg/L IBA+10 g/L AC培养基上生根效果较好，生根率达90%~96.67%.<br>The shoot-tips, mature stem segments with buds, internodes and leaves were used as explants in a study of the tissue culture of jujube (<i>Zizyphus jujuba</i>) Mill. cv. Wulongzhuyaozao. The mature stem segments with buds were shown to be the most suitable explants. In the primary culture, 1/2MS+1 mg/L 6-BA+0.2 mg/L NAA was the best medium, in which the bud sprouted fast, and the rate of adventitious shoot regeneration from the induced calli was 53.3%. The best medium for proliferation was MS+1 mg/L 6-BA+0.2 mg/L IBA, and the bud multiplication coefficient was as high as 3.77. The adventitious shoots rooted readily on 1/2MS+0.3 mg/L IBA+10 g/L activated carbon, with a rooting percentage of 90%-96.67%. The identification of suitable conditions for tissue culture of Wulongzhuyaozao laid a good foundation for virus elimination, rejuvenation and variety improvement of this jujube cultivar
%K 武隆猪腰枣
%K 组织培养
%K 快速繁殖&lt
%K br&gt
%K jujube (&lt
%K i&gt
%K Zizyphus jujuba&lt
%K /i&gt
%K ) Mill. cv. Wulongzhuyaozao
%K tissue culture
%K rapid propagation
%U http://xbgjxt.swu.edu.cn/jsuns/html/jsuns/2017/5/201705006.htm