%0 Journal Article
%T 蛋鸡资源群体的盲肠长度全基因组关联分析<br>Genome-Wide Association Study (GWAS) for Cecum Length in Laying Hens
%A 王星果
%A 曲亮
%A 卢建
%A 窦套存
%A 马猛
%A 胡玉萍
%A 沈曼曼
%A 郭军
%A 李尚民
%A 王克华&lt
%A br&gt
%A WANG Xing-guo
%A QU Liang
%A LU Jian
%A DOU Tao-cun
%A MA Meng
%A HU Yu-ping
%A SHEN Man-man
%A GUO Jun
%A LI Shang-min
%A WANG Ke-hua
%J 西南大学学报(自然科学版)
%D 2018
%R 10.13718/j.cnki.xdzk.2018.09.006
%X <sec>目的盲肠是鸡进一步消化和吸收养分，尤其是纤维的器官，对鸡的生长及后期产蛋都具有重要作用，因此鸡的盲肠长度是一个重要的生理指标.
</sec>



<sec>方法通过对白来航鸡与东乡绿壳蛋鸡杂交所得F2代个体的盲肠长度进行测量，并运用单核苷酸多态性（SNP）芯片检测其基因分型，根据SNP检测数据运用SAS进行遗传评估，并用全基因组混合模型关联算法（GEMMA）进行单变量全基因组关联分析（GWAS）.
</sec>



<sec>结果结果显示，盲肠长度表现出中等遗传力（0.39）.GWAS鉴定出54个SNP与盲肠的长度显著相关，且1号染色体170 Mb附近对于盲肠长度来说是一个重要区域.在这个区域，覆盖26个SNP位点的18个基因被定为候选基因，其中2个分别在编码序列（CDS）和3'非翻译区（3'UTR），对应于<i>NHLRC</i>3和<i>SIAH</i>3，它们可能是影响盲肠长度的重要SNP位点和基因.
</sec>



<sec>结论利用GWAS筛选并鉴定出和鸡盲肠长度相关联的SNP位点及基因，将为揭示蛋鸡盲肠发育的机制和分子育种提供基础.
</sec><br><sec>ObjectiveCecum is a chicken organ for digestion and absorption of nutrients, especially fiber, which plays an important role in the growth and egg production of chicken. So chicken cecum length is an important physiological index. Because studies on cecum length are limited, it is necessary to carry out a study of its genetic structure.
</sec>



<sec>MethodsIn the current study, the cecum length of the F2s of reciprocal crosses between white leghorn and Dongxiang was measured, the genotypes were detected using the SNP (single-nucleotide polymorphism) chip, the genetic evaluation was detected using the SAS according to the SNP data, and univariate analysis of genome-wide association (GWAS) was conducted with genome-wide efficient mixed model (GEMMA) correlation algorithm.
</sec>



<sec>ResultsResults showed that the cecum length exhibited a medium heritability (0.39). By GWAS we identified 54 SNPs significantly associated with cecum length, and about 170 Mb on Chromosome 1 was an important area for cecum length. In this region, 18 genes covering 26 SNPs were classified as candidate genes, two of which were in the coding sequence (CDS) and 3'untranslated region (3'UTR), corresponding to the <i>NHLRC</i>3 and <i>SIAH</i>3, respectively. They might be important SNPs and genes which influenced the cecum length in chicken.
</sec>



<sec>ConclusionThe SNPs and genes associated with cecum length in chicken were identified by GWAS. It will help to provide the basis for understanding the mechanism of cecum development and molecular breeding in laying hens.
</sec
%K 蛋鸡
%K 盲肠长度
%K GWAS
%K 遗传结构&lt
%K br&gt
%K laying hen
%K cecum length
%K GWAS
%K genetic architecture
%U http://xbgjxt.swu.edu.cn/jsuns/html/jsuns/2018/9/20180906.htm