%0 Journal Article
%T 以诺丽茎段为外植体的无籽基因转化研究<br>Studies on Genetic Transformation of Seedlessness Gene to Noni (<i>Morinda citrifolia</i>) with Its Stem Segments as the Explants
%A 贾丹丹
%A 蓝增全
%A 吴田&lt
%A br&gt
%A JIA Dan-dan
%A LAN Zeng-quan
%A WU Tian
%J 西南大学学报(自然科学版)
%D 2018
%R 10.13718/j.cnki.xdzk.2018.09.002
%X 以诺丽无菌苗不带腋芽的茎段为转化材料，采用根癌农杆菌介导法，将含有无籽基因的质粒pCAMBIA1305.1导入到诺丽细胞中，通过Kan快速筛选、PCR检测等方法对转化子进行鉴定.实验结果表明：抗性芽的平均分化频率为42%，经过PCR检测，以茎为外植体进行遗传转化的转化率为5.4%.在转化过程中，有些植株可能会发生饰变.该实验初步证实已把无籽基因转入诺丽，后续需Southern杂交进一步验证.<br>In this study, the method of <i>Agrobacterium</i>-mediation was used to import pCAMBIA 1305.1, which carries the seedlessness gene, into the cells of the stem segments without axillary buds of aseptic noni (<i>Morinda citrifolia</i>). Then we identified the transformant with the methods of Kan quick screening and PCR checking. PCR results showed that the average differentiation rate of resistant buds was 42%, and the conversion rate of the genetic transformation using noni stem segments as the explants was 5.4%. Furthermore, in the process of transformation, modification could occur in some plants. This study preliminarily confirmed that the seedlessness gene had been transformed into the noni plants, but Southern hybridization is to be made in further study to verify the results of this study
%K 海巴戟
%K 茎段
%K 农杆菌
%K 转基因&lt
%K br&gt
%K &lt
%K i&gt
%K Morinda citrifolia&lt
%K /i&gt
%K stem segment
%K &lt
%K i&gt
%K Arobacterium&lt
%K /i&gt
%K genetic transformation
%U http://xbgjxt.swu.edu.cn/jsuns/html/jsuns/2018/9/20180902.htm