%0 Journal Article %T 党参提取物抗运动性疲劳机制研究<br>Research on the Mechanism of Resistance to Exercise-Induced Fatigue of <i>Codonopsis</i> Extract %A 尚尧 %A 李靖文< %A br> %A SHANG Yao %A LI Jing-wen %J 西南大学学报(自然科学版) %D 2018 %R 10.13718/j.cnki.xdzk.2018.06.002 %X 研究中药党参提取物抗运动性疲劳的效应及机制.方法：随机将昆明小鼠分为空白对照组、党参提取物低剂量组(100 mg/(kg·d))、中剂量组(300 mg/(kg·d))、高剂量组(600 mg/(kg·d)).喂养20 d后，检测其负重力竭游泳时间、肝糖原含量、运动后血乳酸、血清尿素氮含量以及腓肠肌PGC-1α蛋白的表达.测量结果：党参提取物能够显著增加昆明小鼠力竭游泳时间，并显著降低血乳酸含量(<i>p</i>＜0.05)；此外高剂量组中血清尿素氮含量降低了28.64%(<i>p</i>＜0.01)，肝糖原含量升高了30.30%(<i>p</i>＜0.01)；Western Blot检测结果显示，高、中、低剂量组PGC-1α表达较空白组均有显著提高(<i>p</i>＜0.05).结论：说明党参提取物很可能是通过提高PGC-1α蛋白的表达来减少代谢产物的堆积，增加糖原含量，从而达到抗运动性疲劳的作用.<br>To study the effect and mechanism of <i>Codonopsis pilosula</i> extract against exercise-induced fatigue. Methods: Kunming mice were randomly divided into blank control group, low dose group (100 mg/(kg·d)), medium dose group (300 mg/(kg·d)) and high dose group (600 mg/(kg·d)). After feeding 20 d, their exhaustive swimming time, liver glycogen content, blood lactic acid content, serum urea nitrogen and the expression of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein in the gastrocnastus were measured. The results showed that the extract of <i>C. pilosula</i> significantly increased the exhaustive swimming time and significantly decreased the content of blood lactate in Kunming mice (<i>p</i> < 0.05). In addition, the content of serum urea nitrogen in the high dose group decreased by 28.64% (<i>p</i> < 0.01). The content of PGC-1α increased by 30.30% (<i>p</i> < 0.01). The results of Western Blot showed that the expression of PGC-1α in the high, medium and low dose groups significantly increased compared with that of the blank group (<i>p</i> < 0.05). Conclusion: <i>C. pilosula</i> extract may play a role in resistance to exercise-induced fatigue by increasing the expression of PGC-1α protein, reducing the accumulation of metabolites and increasing the content of glycogen %K 党参 %K 运动性疲劳 %K PGC-1α< %K br> %K < %K i> %K Codonopsis pilosula< %K /i> %K (Franch.) Nannf. %K exercise-induced fatigue %K PGC-1α %U http://xbgjxt.swu.edu.cn/jsuns/html/jsuns/2018/6/201806002.htm