%0 Journal Article
%T 青蒿&lt;i&gt;HMGR&lt;/i&gt;基因家族克隆及对茉莉酸甲酯和损伤的响应&lt;br&gt;Molecular Cloning and Expression Analysis of HMG-CoA (3-hydroxy-3-methylglutaryl-CoA) Reductase Gene Family Upon MeJA and Mechanical Injury Treatments in &lt;i&gt;Artemisia annua&lt;/i&gt; L.
%A 陈俞裴
%A 申甜
%A 邱飞
%A 陈敏
%A 廖志华
%A 兰小中
%A 杨春贤&lt
%A br&gt
%A CHEN Yu-pei
%A SHEN Tian
%A QIU Fei
%A CHEN Min
%A LIAO Zhi-hua
%A LAN Xiao-zhong
%A YANG Chun-xian
%J 西南大学学报(自然科学版)
%D 2017
%R 10.13718/j.cnki.xdzk.2017.12.007
%X 通过全基因组序列测定和RACE技术, 从青蒿(&lt;i&gt;Artemisia annua&lt;/i&gt; L.)克隆出了3条编码&lt;i&gt;HMGR&lt;/i&gt;的cDNA序列:&lt;i&gt;AaHMGR&lt;/i&gt;1, &lt;i&gt;AaHMGR&lt;/i&gt;2和&lt;i&gt;AaHMGR&lt;/i&gt;3, 并对3条基因进行生物信息学分析.结果表明:3条序列与其他物种的&lt;i&gt;HMGR&lt;/i&gt;具有高度相似性但基因组结构不一致; 组织表达分析中, AaHMGR1在茎中表达量最高, 而&lt;i&gt;AaHMGR&lt;/i&gt;2和&lt;i&gt;AaHMGR&lt;/i&gt;3在花中表达量较高; 外源茉莉酸甲酯(MeJA)诱导处理后&lt;i&gt;AaHMGR&lt;/i&gt;1的表达在0.5 h时达到最大值, &lt;i&gt;AaHMGR&lt;/i&gt;2和&lt;i&gt;AaHMGR&lt;/i&gt;3则轻微响应; 机械损伤3 h后能够使&lt;i&gt;AaHMGR&lt;/i&gt;1的表达量上调约700倍, 而&lt;i&gt;AaHMGR&lt;/i&gt;2, &lt;i&gt;AaHMGR&lt;/i&gt;3上调幅度却不大.综上研究结果表明, &lt;i&gt;AaHMGR&lt;/i&gt;1基因可能在青蒿素的生物合成中起着更为重要的作用.&lt;br&gt;Three full-length cDNA of HMGR, i.e. &lt;i&gt;AaHMGR&lt;/i&gt;1, &lt;i&gt;AaHMGR&lt;/i&gt;2 and &lt;i&gt;AaHMGR&lt;/i&gt;3, were cloned from &lt;i&gt;Artemisia annua&lt;/i&gt;. Bioinformatic and phylogenetic analyses revealed that all the three &lt;i&gt;AaHMGRs&lt;/i&gt; shared extensive homology with other plant species and belonged to the plant HMGR family; however, the corresponding coding region of the genome structure was different. The expression of &lt;i&gt;AaHMGR&lt;/i&gt;1 was the highest in the stem, while the expression of &lt;i&gt;AaHMGR&lt;/i&gt;2 and &lt;i&gt;AaHMGR&lt;/i&gt;3 was relatively high in the flower. The expression of &lt;i&gt;AaHMGR&lt;/i&gt;1 reached the maximum at 0.5 h after MeJA induction, and then decreased gradually, while &lt;i&gt;AaHMGR&lt;/i&gt;2 and &lt;i&gt;AaHMGR&lt;/i&gt;3 responded but slightly. The expression of &lt;i&gt;AaHMGR&lt;/i&gt;1 increased by about 700 times at 3 h after mechanical injury, while that of &lt;i&gt;AaHMGR&lt;/i&gt;2 and &lt;i&gt;AaHMGR&lt;/i&gt;3 was not significant. The above results suggested that &lt;i&gt;AaHMGR&lt;/i&gt;1 may play a crucial role in artemisinin biosynthesis
%K 青蒿
%K &lt
%K i&gt
%K HMGR&lt
%K /i&gt
%K MeJA
%K 损伤
%K 青蒿素&lt
%K br&gt
%K &lt
%K i&gt
%K Artemisia annua&lt
%K /i&gt
%K HMGR
%K MeJA
%K wound
%K artemisinin
%U http://xbgjxt.swu.edu.cn/jsuns/html/jsuns/2017/12/201712007.htm