%0 Journal Article %T 水稻microRNA及其靶基因的系统鉴定<br>Systematic identification of miRNAs and miRNA targets in Oryza sativa %A 马轩 莫蓓莘 曹晓风 %J 华南师范大学学报(自然科学版) %D 2017 %X miRNA在植物生长发育和环境胁迫中发挥着重要的作用.本研究基于49个水稻小RNA 测序数据,系统分析了已知miRNA的表达,并鉴定到了新的水稻miRNA.我们发现,miRBase上注释的水稻miRNA中,只有39%具有可检测的表达. 此外,对水稻降解组数据的分析鉴定到一些新的miRNA靶基因,包括两个新的miR444靶基因,它们分别编码WD40蛋白和热休克因子型DNA结合蛋白.<br>:miRNAs play essential roles in plant development and environmental adaption. Here, based on 49 Oryza sativa (rice) small RNA-seq datasets, we systematically analyzed the expressions of known miRNAs and identified novel miRNAs in rice. We showed that only 39% of annotated miRNAs on miRBase have detectable expressions. Additionally, analysis of rice degradome datasets identified a few new miRNA targets including two miR444 targets that encode a WD40 protein and a HSF (heat shock factor)-type DNA-binding protein, respectively %K 水稻 %K 小RNA测序 %K 降解组 %K miRNA %K 靶基因 %K < %K br> %K rice %K small RNA-seq %K degradome %K miRNA %K targets %U http://journal.scnu.edu.cn:8080/jwk_xbzrb/CN/abstract/abstract4132.shtml