%0 Journal Article
%T Optimization and Validation of RP-HPLC-UV/Vis Method for Determination Phenolic Compounds in Several Personal Care Products
%A Mohammed Akkbik
%A Zaini Bin Assim
%A Fasihuddin Badruddin Ahmad
%J International Journal of Analytical Chemistry
%D 2011
%I Hindawi Publishing Corporation
%R 10.1155/2011/858153
%X An HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methyl cinnamate (OMC) as UVB-filter in several personal care products. The dynamic range was between 1 to 250ˋmg/L with relative standard deviation less than 0.25% ( ). Limits of detection for BHA, BHT, and OMC were 0.196, 0.170, and 0.478ˋmg/L, respectively. While limits of quantification for BHA, BHT, and OMC were 0.593, 0.515, and 1.448ˋmg/L, respectively. The recovery for BHA, BHT, and OMC was ranged from 92.1每105.9%, 83.2每108.9%, and 87.3每103.7%, respectively. The concentration ranges of BHA, BHT, and OMC in 12 commercial personal care samples were 0.13每4.85, 0.16每2.30, and 0.12每65.5ˋmg/g, respectively. The concentrations of phenolic compounds in these personal care samples were below than maximum allowable concentration in personal care formulation, that is, 0.0004每10ˋmg/g, 0.002每5ˋmg/g, and up to 100ˋmg/g for BHA, BHT, and OMC, respectively. 1. Introduction Phenolic compounds such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) act as antioxidants and octyl methyl cinnamate (OMC) as UVB-filter are active compounds in personal care products (see Figure 1) [1, 2]. Figure 1: Structures of common phenolic compounds in personal care products. BHA and BHT are addedsingly or in combination to prevent oxidative rancidity in personal care products [3]. While octyl methyl cinnamate (OMC) is used to absorb the dangerous UV-light between 280每320ˋmmto and to protect the skin from sunburn [2]. The concentration of BHA and BHT in personal care formulation depends on the amount of sensitive compounds (alpha hydroxy acids, ceramides, lipids, vitamins, oils, and so forth) that are susceptible to oxidation by the oxygen in the atmosphere making it possible for the unstable peroxide radicals [4, 5]. BHA and BHT are able to inhibit reactions promoted by oxygen, thus avoiding the oxidation and are intended to prevent the appearance of ketones and aldehydes that can give a product a disagreeable smell and rancidity [5]. To prevent cosmetic formulations from peroxide radicals we must use antioxidant compounds which have the ability to neutralize those radicals through the transfer of hydrogen to this radical, stabilizing the antioxidant by resonance [6, 7]. While the concentration of OMC depends on the type of product and part of body it is applied on (face, hand, lips, and other parts of
%U http://www.hindawi.com/journals/ijac/2011/858153/