%0 Journal Article
%T 无标记定量蛋白质组学分析AMACR过表达对肝癌细胞生物学行为的影响<br>Label-free quantitative proteomic analysis of the effect of AMACR overexpression on biological behaviors of hepatocellular carcinoma cells
%A 夏江宝
%A 邢晓华
%A 王英超
%A 沈佐君
%J 福州大学学报(自然科学版)
%D 2016
%R 10.7631/issn.1000-2243.2016.02.0282
%X 研究α-甲酰基辅酶A消旋酶（AMACR）过表达对肝癌细胞生物学行为的影响及其分子机制. 首先建立AMACR稳定过表达细胞株，然后提取AMACR过表达细胞株的全蛋白，进行无标记定量蛋白质组学研究，最后对鉴定结果进行生物信息学分析和结果验证，共筛选出138种差异表达蛋白. 这些差异表达蛋白主要参与代谢加工、细胞加工等，证明AMACR的过表达对于肝癌细胞的生物学行为影响巨大. IPA分析发现，这些差异表达蛋白主要参与了ERK1/2信号通路和NF-κB信号通路. 以上结果说明，AMACR的过表达通过调节ERK1/2和NF-κB信号通路等手段改变肝癌细胞的生物学行为.<br>This study attempted to investigate the effect of AMACR overexpression on the biological behavior of HCC cells using label free quantitative proteomic approch. A stable cell line that overexpressed AMACR was produced by transducing engineered lentivirus containing AMACR complete sequence into HepG2 cells. Afterwards，the whole protein extracted from the AMACR-overexpressed cells and the normal cultured cells (control) were comparatively quantified by 2D LC-MS/MS. A total of 138 differentially expressed proteins were identified. These dysregulated proteins were mostly enriched for metabolic process and cellular process，which suggested a big change at biological behaviors occurred in the host cells due to AMACR overexpression. The signalings pathway analysis revealed that the dysregulated proteins in AMACR-overexpressed cells are more concentrated to the ERK1/2 and NF-κB signaling pathways. Thus，AMACR overexpression induced the alterations at biological behaviors of HCC cells majorly through modulating the ERK1/2 and NF-κB signalings
%K 肝细胞癌 α-甲酰基辅酶A消旋酶 细胞增殖 无标记定量蛋白质组学&lt
%K br&gt
%K hepatocellular carcinoma AMACR cell proliferation label-free quantitative proteomics
%U http://xbzrb.fzu.edu.cn/ch/reader/view_abstract.aspx?file_no=201602023&flag=1