%0 Journal Article
%T 基于MoS2模拟酶快速比色检测葡萄酒中的亚硫酸根<br>Rapid colorimetric detection of sulfite root in wine based on MoS2 simulation enzyme
%A 陈清爱
%A 宋志平
%A 郭良洽
%A 庞
%A 杰
%J 福州大学学报(自然科学版)
%D 2017
%R 10.7631/issn.1000-2243.2017.03.0432
%X MoS2纳米片能够有效地催化过氧化氢氧化底物和3，3′，5，5′-四甲基联苯胺（TMB），产生颜色变化，利用亚硫酸根能够消耗过氧化氢而降低反应体系吸光度的原理，建立一种亚硫酸根的快速比色传感体系并用于葡萄酒样品的检测. 实验考察并优化了反应时间、H2O2浓度、pH值和温度等影响参数，在最优条件下（反应时间30 min、H2O2 浓度100 μmol·L-1、pH值7.0、温度25 ℃），亚硫酸根浓度在5.0 ~120.0 μmol·L-1范围内与吸光度具有良好的线性关系，检测限（3σ/k）为0.5 μmol·L-1. 采用所建立的方法测定葡萄酒中的亚硫酸根，样品加标回收率为96.6%~106.5%.<br>Molybdenum disulfide (MoS2) can catalytically oxidize 3，3′，5，5′-tetramethylbenzidine (TMB) by H2O2 to produce a blue color product，and the absorbance of system can reduce due to the consumption of H2O2 by sulfite root. Based on this principle，a simple colorimetric method for sulfite root detection was developed and applied to detect sulfite in wine samples. Several analytical parameters including reaction time，the concentration of H2O2，pH value and temperature were investigated and optimized. Under the optimal conditions (reaction time 30 min，H2O2 100 μmol·L-1，pH 7.0 and temperature 25 ℃)，a linear relationship between the concentration of sulfite and absorbance could be obtained over the range of 5.0 to 120.0 μmol·L-1 with a detection limit of 0.5 μmol·L- (3σ/k). This method was applied to detect sulfite root in white grape wine with the recoveries of 96.6%-106.5%
%K 二硫化钼 亚硫酸根 过氧化氢 葡萄酒&lt
%K br&gt
%K molybdenum disulfide (MoS2) sulfite root H2O2 wine
%U http://xbzrb.fzu.edu.cn/ch/reader/view_abstract.aspx?file_no=201703022&flag=1