%0 Journal Article
%T 水稻miR446及其靶基因APP1在非生物胁迫中 的功能研究<br>Function analysis of Osa-miR446 and its target gene APP1 in abiotic stresses
%A 廖雪洪
%A 艾涛波
%A 王胜华
%J 四川大学学报 (自然科学版)
%D 2016
%X 根据基因芯片的数据筛选出Osa-miR446及靶基因作为研究对象。生物信息学分析表明，Osa-miR446有4个候选靶基因，通过RACE技术对这些候选靶基因进行鉴定，发现只有LOC_Os03g56930.1（APP1基因）可得到理想的剪切条带，测序后比对可知该剪切位点位于APP1基因的内含子区域（第1036-1037个碱基）。随后，通过克隆获得APP1基因，信息学分析可知该基因开放阅读框长度为711bp，是一个编码237个氨基酸的新的未定性的疏水性蛋白质。Southern杂交结果显示APP1在水稻基因组中为单拷贝基因。 利用荧光定量PCR分析了Osa-miR446及其靶基因APP1在非生物胁迫中的表达模式，结果表明Osa-miR446与APP1基因的表达均呈负相关，说明Osa-miR446确实可通过剪切APP1基因的转录产物来对非生物胁迫应答。<br>We researched Osa-miR446 and its target gene APP1 on the basis of gene microarray. According to bioinformatics analysis, Osa-miR446 might have four target genes, but only LOC_Os03g56930.1 (named APP1) could be identified as one of target genes of Osa-miR446 via the RACE experiment. APP1 had an open reading frame of 711bp，encoding an undefined hydrophobicity protein with 237 amino acids. The Southern experiment showed that APP1 was a single-copy gene in rice genome. Expression profile under stressed of Osa-miR446 and APP1 was analyzed. Overall, the results indicated miR446 could regulate APP1 via splicing the transcriptions under stresses and they both played roles in stress response
%K 水稻 miR446 靶基因 APP1 胁迫&lt
%K br&gt
%K Rice miR446 Target gene APP1 Stress
%U http://science.ijournals.cn/jsunature_cn/ch/reader/view_abstract.aspx?file_no=B150228&flag=1