%0 Journal Article
%T miR-330调控TAp73α介导的结直肠癌细胞HCT116对顺铂的敏感性<br>TAp73α-mediated cisplatin sensitivity was suppressed by miR-330 in colorectal cancer cell HCT116
%A 黄玲
%A 童英
%A 肖智雄
%A 张渝君
%J 四川大学学报 (自然科学版)
%D 2016
%X p73是p53基因家族中的一员，参与了细胞周期进程、凋亡和肿瘤耐药性等过程的调控.我们发现，在结肠癌细胞HCT116中，沉默p73可促进细胞对顺铂的敏感性，而且这种作用不依赖于p53.Hsa-miR-330 (miR-330)可直接抑制细胞内源性p73的表达并促进其对顺铂的敏感性.p73则可拮抗miR-330诱导的顺铂敏感性.我们的结果表明了p73对细胞顺铂敏感性的调控作用，为治疗顺铂抗性的肿瘤细胞提供了一条新途径.<br>The p53 family member p73 is a transcription factor involved in the regulation of cell cycle, apoptosis and cancer cell drug-resistant. Here we reported that knockdown of p73 sensitized HCT116 colorectal cancer cells to cisplatin treatment independent of p53. Hsa-miR-330 (miR-330) was identified to be able to directly repress the expression of TAp73α. Overexpression of miR-330 decreased the protein levels of endogenous TAp73α and phenocopied the effect of p73 knockdown. Restoration of TAp73α eliminated miR-330-induced chemosensitivity toward cisplatin. Our results demonstrated a novel function of TAp73α to impair cisplatin sensitivity in colorectal cancer cells which can be repressed by miR-330, thus provided an effective strategy for therapeutic treatment of cisplatin-resistant cancer cells
%K p73 miR-330 细胞凋亡 顺铂 结肠癌&lt
%K br&gt
%K P73 MiR-330 Apoptosis Cisplatin Colorectal cancer
%U http://science.ijournals.cn/jsunature_cn/ch/reader/view_abstract.aspx?file_no=B150167&flag=1