%0 Journal Article
%T AtMYB44与ABI1竞争性结合ABA受体RCAR1的研究<br>The research of AtMYB44 and ABI1 via competitive binding to ABA receptor RCAR1 in ？？Arabidopsis thaliana？？
%A 赵哲
%A 李德款
%A 袁德志
%A 王晓玉
%A 李旭锋
%J 四川大学学报 (自然科学版)
%D 2015
%X 为了进一步确定AtMYB44、ABI1、RCAR1三者之间的关系，本文进行了竞争性pull down和ABI1磷酸酶活性的测定实验，结果表明AtMYB44与ABI1能够竞争性结合RCAR1，并且这种竞争性结合依赖于ABA，AtMYB44能够降低RCAR1对ABI1活性的抑制，这为深入研究ABA信号转导途径奠定了基础.<br>In order to determine the relationship among the AtMYB44,ABI1 and RCAR1, competitive GST pull down assay and ABI1 phosphatase activity assay were used in this study, these results suggested that AtMYB44 and ABI1 compete ？？in vitro？？ for binding to RCAR1 in the presence of ABA. In addition, AtMYB44 reduces the inhibitory effect of RCAR1 on ABI1 phosphatase activity in the presence of ABA, which build the foundation for the ABA signal pathway study
%K 竞争性蛋白沉降技术 RCAR1 ABI1 AtMYB44？？&lt
%K br&gt
%K Competitive pull down RCAR1 ABI1 AtMYB44
%U http://science.ijournals.cn/jsunature_cn/ch/reader/view_abstract.aspx?file_no=20153034&flag=1