%0 Journal Article %T 新疆河狸mtDNA D-loop HV-Ⅰ区的遗传多样性研究<br>Research on genetic diversities of Asiatic beaver (Castor fiber birulai)on mtDNA D-loop HV-Ⅰin Xinjiang, China %A 杜聪聪 %A 赵晶 %A 初红军 %A 刘元超 %A 端肖楠 %A 陈刚< %A br> %A DU Cong-cong %A ZHAO Jing %A CHU Hong-jun %A LIU Yuan-chao %A DUAN Xiao-nan %A CHEN Gang %J 山东大学学报（理学版） %D 2016 %R 10.6040/j.issn.1671-9352.0.2015.458 %X 摘要： 通过测定和分析我国新疆乌伦古河流域16只河狸的mtDNA D-loop HV-Ⅰ区序列,对新疆河狸的遗传多样性进行了研究。结果表明,新疆河狸mtDNA D-loop HV-Ⅰ区序列长度为569 bp, A、T、G和C四种核苷酸的平均比例分别为27.0%、32.7%、24.0%和16.2%,新疆河狸mtDNA D-loop HV-Ⅰ区A+T含量高于G+C含量,表现出碱基组成的偏倚性。与蒙古国河狸mtDNA D-loop HV-Ⅰ区(AY623632)比对后,发现我国新疆河狸mtDNA D-loop HV-Ⅰ区10个变异位点,占分析位点总数的2.03%,其中转换位点4个,颠换位点5个,缺失位点1个,无插入位点。依据GenBank上已公布的不同地区河狸种群mtDNA D-loop HV-Ⅰ区序列,构建系统树后发现我国新疆河狸和蒙古国河狸位于一个分支,说明两者属于欧亚河狸种下的同一个亚种-蒙新亚种(Castor fiber birulai)。在16只新疆河狸个体中鉴定出2种单倍型,单倍型多样性(Hd)为0.233 00±0.015 78,核苷酸多样性(Pi)为0.001 23±0.015 90,平均核苷酸差异数(K)为0.700,与其他河狸亚种种群比较后发现新疆河狸遗传多样性较低,基因丰富度低,这与新疆河狸局限的生活环境和人为因素的影响是密切相关的。<br>Abstract: Genetic diversities of Asiatic beaver(Castor fiber)in Xinjiang was investigated by analyzing the HV-Ⅰ sequences of the mitochondrial DNA control region(D-loop)of 16 individuals from the Ulungur watershed of Xinjiang in China. Results showed that the length of mtDNA D-loop HV-Ⅰ sequence of Xinjiang beaver was 569 bp, with nucleotide frequencies of 27.0%,32.7%,24.0% and 16.2% for A,T,G and C respectively, and the frequencies of A and T 山 东 大 学 学 报 (理 学 版)第51卷 - 第3期杜聪聪,等:新疆河狸mtDNA D-loop HV-Ⅰ区的遗传多样性研究 \=-was higher than that of G and C, which indicated the preference of nucleotides; that 10 polymorphic sites(2.03% of total analyzed sites)were found in the mtDNA D-loop HV-Ⅰ sequence of Xinjiang beaver compared with that(AY623632)of Mongolia beaver, of which there were four transitions sites, five transversions sites and one missing site; that Xinjiang beaver and Mongolia beaver were in one cluster by phylogenetic analyses based on the open mtDNA D-loop HV-Ⅰ sequence of beaver in other regions on GenBank, suggesting Xinjiang beaver also belonged to the subspecies of C. f. birulai; that Xinjiang beaver population had two unique haplotypes, whose haplotype diversity(Hd), nucleotide diversity(Pi)and average number of nucleotide differences(K)were 0.233 00±0.015 78, 0.001 23±0.015 90 and 0.700, indicating a low level of genetic diversity, deeply related to its limited environment and human factors %K mtDNA D-loop HV-Ⅰ区 %K 遗传多样性 %K 新疆河狸 %K < %K br> %K Xinjiang beaver %K genetic diversity %K mtDNA D-loop %U http://lxbwk.njournal.sdu.edu.cn/CN/10.6040/j.issn.1671-9352.0.2015.458