%0 Journal Article %T 卤虫(AC) n和(AG) n微卫星文库构建<br>Construction of (AC) n and (AG) n Microsatellite DNA Libraries in Brine Shrimp Artemia %A 刘海珍 %A 邹小艳 %A 郭松长 %A 李双 %A 张湑泽 %A 马建滨 %A 都玉蓉 %J 四川动物 %D 2016 %X 中文摘要：卤虫 Artemia作为重要的基础实验材料和经济动物资源,其微卫星位点具有重要的研究和应用价值。本研究使用磁珠富集法,成功构建了卤虫的(AC) n和(AG) n微卫星富集文库。通过对文库进行PCR方法鉴定和测序确认,所构建的(AC) n和(AG) n文库阳性克隆率分别为55.8%和34.5%。依据得到的微卫星序列,设计并合成了63对微卫星引物,随机以2个地理单元(青海、西藏)共18个卤虫基因组DNA为扩增模板,筛选出6对具有多态性的卤虫微卫星引物。本研究筛选出的微卫星多态位点可用于卤虫种群的遗传多样性评估、遗传图谱的构建和数量性状定位等后续工作。<br>英文摘要：Brine shrimp Artemia is an important model organism and economic animal resource. The microsatellite loci of this species have a significant research and application value. In this study, the Artemia microsatellite enriched DNA libraries of (AC) n and (AG) n were conducted successfully using the method of FIASCO (fast isolation by AFLP sequence containing repeats). In the (AC) n library, 67 colonies (total no. 120) were picked out and sequenced, with a positive clone rate of 55.8%. In the (AG) n library, 69 colonies out of 200 colonies were sequenced, with a positive clone rate of 34.5%. Among all the sequences containing microsatellites, 63 pairs of primers were designed and synthesized for further primer screening. By using 18 Artemia DNA samples from Qinghai and Tibetan, 6 pairs of primers with polymorphic microsatellite loci of Artemia were identified. These polymorphic loci may facilitate further study on the detection of genetic diversity, genetic linkage mapping and the location of quantitative traits of Artemia. 2016,(35): 356-360 收稿日期：2015-10-13 DOI：10.11984/j.issn.1000-7083.20150317 分类号：Q959.223;Q78 基金项目：青海省应用基础研究计划项目(2013-Z-750;2013-Z-751) 作者简介：刘海珍(1989-),女,硕士研究生 *通讯作者：马建滨,E-mail:mjb_117@163.com;都玉蓉,xndyr@163.com 参考文献： 都玉蓉, 马建滨, 徐永涛, 等. 2014. 藏羚羊(AC) n ,(AG) n 微卫星文库构建[J]. 四川动物, 33(1):19-22. 李强, 万建民. 2005. SSRHunter, 一个本地化的SSR位点搜索软件的开发[J]. 遗传, 27(5):808-810. 马志珍, 陈汇远, 武振彬, 等. 1996. 中国内陆盐湖卤虫资源开发利用研究[J]. 海洋水产研究, 17(1):1-14. 马志珍. 1993. 中国卤虫生物地理分布的研究[J]. 现代渔业信息, 8(9):19-26. 印象初, 印红, 周可新, 等. 2001. 高原盐湖盐藻和卤虫资源的开发和利用[J]. 盐湖研究, 9(1):4-8. 袁慧, 张修月, 宋昭彬, 等. 2008. 岩原鲤微卫星富集文库的构建及微卫星分子标记[J]. 四川动物, 27(2):210-215. 郑绵平. 1995. 论"盐湖农业"[J]. 地球科学, 4:404-418. 郑绵平. 2001. 青藏高原盐湖资源研究的新进展[J]. 地球科学, 22:97-102. Carleton KL, Streelman JT, Lee BY, et al . 2002. Rapid isolation of CA microsatellites from the tilapia genome[J]. Animal Genetics, 33(2):140-144. Sambrook JW, Russell D. 2001. Molecular cloning:a laboratory manual, 3rd ed[M]. New York:Cold Spring Harbor Laboratory Press:463-470. Weber JL. 1990. Informativeness of human (dC-dA) n ·(dG-dT) n polymorphisms[J]. Genomics, 7(4):524-530. Zane L, Bargelloni L, Patarnello T. 2002. Strategies for microsatellite isolation:a review[J]. Molecular Ecology, 11(1):1-16 %K 卤虫 %K 微卫星 %K 富集文库< %K br> %K 英文关键字： Artemia %K microsatellite %K enriched library %U http://www.scdwzz.com/viewmulu.aspx?qi_id=1571&mid=48802&xuhao=7