%0 Journal Article %T 褪黑素对邻苯二甲酸(2-乙基己基)酯致雄性小鼠生殖细胞DNA损伤的拮抗作用<br>Antagonism of Melatonin against Di-(2-ethylhexyl) Phthalate-induced DNA Damage in Mice Testicular Cells %A 黄文捷 %A 冯为 %A 李阳 %A 陈于 %J 四川动物 %D 2015 %X 中文摘要： 目的 探讨邻苯二甲酸(2-乙基己基)酯(DEHP)致小鼠睾丸细胞DNA损伤及褪黑素(MT)对此损伤的拮抗作用。 方法 将40只CL57BL/6J雄性小鼠随机分为4组,包括对照组、MT组、DEHP组和MT+DEHP联合组。MT采用腹腔注射(剂量为15 mg·kg -1),DEHP灌胃染毒(染毒剂量为1000 mg·kg -1),每天染毒1次,连续30 d。检测睾丸组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活力和丙二醛(MDA)、8-羟基脱氧鸟嘌呤(8-OHdG)含量。单细胞凝胶电泳(彗星实验)检测睾丸细胞DNA损伤,慧星图像软件测定慧星尾长、慧尾DNA百分含量、尾矩及Olive尾矩。 结果 与对照组比较,DEHP组小鼠睾丸组织GSH-Px和SOD活力降低,MDA和8-OHdG 含量增加,睾丸细胞彗星尾长、彗尾DNA百分含量、尾矩、Olive尾矩均显著增加,差异均有统计学意义( P<0.05);与DEHP组比较,MT+DEHP联合组小鼠睾丸组织GSH-Px和SOD活力升高,MDA和8-OHdG含量降低,睾丸细胞DNA损伤程度减轻,差异均有统计学意义( P<0.05)。 结论 DEHP造成小鼠睾丸明显的氧化应激,并引起睾丸细胞DNA的损伤;MT可拮抗因DEHP染毒导致的睾丸氧化损伤。<br>英文摘要： Objective To investigate the antagonistic effect of melatonin against di-(2-ethylhexyl) phthalate (DEHP)-induced DNA damage in mice testicular cells. Methods A total of forty CL57BL/6J mice were randomly divided into 4 groups: control (saline) group, MT (melatonin) treated group, DEHP treated group and MT+DEHP treated group. MT was intraperitoneally injected at the dose of 15 mg·kg -1, and DEHP was orally administrated at the dose of 1000 mg·kg -1 for 30 days. Subsequently, the activities of testicular glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), the content of testicular malondialdehyde (MDA) and 8-hydrxydeoxyguanosine (8-OHdG) were measured. The comet assay was applied to measure the DNA damage. Comet Assay Software Project was conducted to analyze the DNA damage (comet tail length, tail DNA%, tail moment and Olive tail moment) of testicular cells. Results Compared with the control group, the contents of MDA and 8-OHdG in testis tissues were significantly increased; and the activities of GSH-Px and SOD were significantly decreased ( P<0.05); the comet tail length, the percentage of tail DNA, tail moment and Olive tail moment in testicular cells were significantly increased in DEHP group ( P<0.05). Compared with the DEHP group, the contents of MDA and 8-OHdG in testis tissues were significantly decreased, the activities of GSH-Px and SOD were significantly increased ( P<0.05); and the DNA damage of testicular cells significantly decreased in MT+DEHP group ( P<0.05). Conclusion DEHP could cause oxidative stress in testis, and induce DNA damage in mice testicular cells. MT could antagonize DEHP-induced oxidative damage in testis of mice. 2015,34(4): 511-517 收稿日期：2014-10-3 DOI：10.11984/j.issn.1000-7083.2015.04.006 分类号：R114;R994.6 作者简介：黄文捷(1989—),女,硕士研究生,研究方向:职业人群健康监护和健康促进,E-mail:aibivicky@163.com *通讯作者：陈于,教授,E-mail:chenyu@cqmu.edu.cn 参考文献： 蔡红平, 肖雯, 朱静, 等. 2011. 邻苯二甲酸二乙基己基酯对大鼠睾丸细胞DNA 损伤和睾丸组织氧化应激影响[J]. 中国职业医学, 38(6): 458-462. 崔月美, 叶建新, 吴茂聪, 等. 2009. %K 邻苯二甲酸(2-乙基己基)酯(DEHP) %K 褪黑素 %K DNA损伤 %K 氧化损伤< %K br> %K 英文关键字：di-(2-ethylhexyl) phthalate (DEHP) %K melatonin %K DNA damage %K oxidative damage %U http://www.scdwzz.com/viewmulu.aspx?qi_id=1456&mid=45379&xuhao=6