%0 Journal Article
%T 基于 COⅠ基因的中国广头叶蝉亚科系统发育初探<br>A Primary Phylogeny Analysis of Macropsinae from China Based on COⅠ Gene
%A 李虎
%A 戴仁怀
%J 四川动物
%D 2018
%X 中文摘要：广头叶蝉亚科Macropsinae广泛分布于世界各地，其内部分类系统较混乱，部分族属级分类地位一直存在争议。本文选取23种叶蝉样本（内群22种+外群1种），分析其线粒体 COⅠ基因部分片段的序列组成和遗传距离，基于最大似然法和贝叶斯法构建广头叶蝉亚科系统发育树。结果发现， COⅠ基因片段序列A+T含量（68.3%）明显较G+C含量（31.7%）高；遗传距离在种间、属间和亚科间的差异有统计学意义；系统发育树较好地支持了横皱叶蝉属 Oncopsis和尖尾叶蝉属 Pedionis的单系性，但广头叶蝉属 Macropsis和暗纹叶蝉属 Pediopsoides较为混乱。<br>英文摘要：Macropsinae is widely distributed around the world. The phylogenetic relationship of the species in Macropsinae appears to be confused, and the taxonomic status of some genera strongly needs to be verified and discussed. 23 Cicadellidae species (including 22 Macropsinae of ingroup and 1 Idiocerinae of outgroup) were chosen as the materials and 23 DNA sequences of mitochondrial COⅠ gene were obtained. The sequence composition and genetic distance of these genes were analyzed, and the phylogenetic trees were built using the methods of Maximum Likelyhood and Bayesian Inference. The results showed that the content of A+T (68.3%) in the sequences of COⅠ gene was significantly higher than that of G+C (31.7%). There were statistically significant differences in the genetic distance among species, genera and subfamilies. The phylogenetic tree well supported the monophyly of genus Oncopsis and Pedionis, but weakly supported that of Macropsis and Pediopsoides. 2018,37(5): 569-574 收稿日期：2018-04-20 DOI：10.11984/j.issn.1000-7083.20180125 分类号：Q953 基金项目：陕西省教育厅自然科学重点科学研究计划项目（16JS020）；陕西省高校科协青年人才托举计划项目（20170209）；中国博士后科学基金资助项目（2016M590968）；陕西理工大学科研基金项目（SLGKYQD2-17，SLGBH16-02）；国家自然科学基金（31000952，3167120382） 作者简介：李虎(1986-),男,博士,讲师,从事昆虫系统学与资源昆虫学研究,E-mail:lc817128hl@163.com *通讯作者：戴仁怀,E-mail:rhdai69@163.com 参考文献： 朴美花, 陈学新, 何俊华. 2002. 膜翅目昆虫干标本的基因组DNA提取[J]. 动物分类学报, 27(4):672-676. 魏重生, 蔡平. 1998. 广头叶蝉属二新种(同翅目:叶蝉科:广头叶蝉亚科)[J]. 昆虫分类学报, 20(2):119-122. Dietrich CH. 2005. Keys to the families of Cicadomorpha and subfamilies and tribes of Cicadellidae (Hemiptera:Auchenorrhyncha)[J]. Florida Entomologist, 88(4):502-517. Evans JW. 1937. Australian leaf-hoppers (Jassoidea, Homoptera):part 4 (Ledridae, Ulopidae and Euscelidae, Paradorydiini)[J]. Papers and Proceedings of the Royal Society of Tasmania, 1936:1-36. Evans JW. 1946. A natural classification of leafhoppers (Jassoidea, Homoptera). Part 1. External morphology and systematic position[J]. Transactions of the Royal Entomological Society of London, 96(3):47-60. Folmer O, Black M, Hoeh W, et al. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit Ⅰ from diverse metazoan invertebrates[J]. Molecular Marine Biology and Biotechnology, 3:294-299. Hamilton KGA. 1980. Contributions to the study of the worldMacropsini
%K COⅠ基因
%K 广头叶蝉亚科
%K 半翅目
%K 系统发育
%K 中国&lt
%K br&gt
%K 英文关键字： COⅠ gene
%K Macropsinae
%K Hemiptera
%K phylogeny
%K China
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