%0 Journal Article %T 川陕哲罗鲑精子超微结构及形态学研究<br>Study on the Morphology and Ultrastructure of Hucho bleekeri Kimura Spermatozoa %A 郭威 %A 厉萍 %A 杨焕超 %A 杜浩 %A 吴金明 %A 危起伟 %J 四川动物 %D 2016 %X 中文摘要：对川陕哲罗鲑 Hucho bleekeri Kimura精子采用扫描电子显微镜及透射电子显微镜进行观察，结果显示：精子由头部、中片和尾部组成；精子全长41.07 μm±2.18 μm，头部长2.76 μm±0.15 μm，头部前端和后端的宽度分别为1.88 μm±0.18 μm和2.08 μm±0.20 μm；尾部长34.74 μm±5.01 μm。头部呈卵圆形，无顶体，主要由细胞核组成；中片由1个不规则的圆球状线粒体及袖套结构组成；线粒体直径为0.82 μm±0.08 μm；尾部呈细长形，并由一个过渡区域分为前端和末端，尾部内部主要由轴丝组成，轴丝为典型的“9+2”结构，外部有不对称性分布的侧鳍结构。结果表明，川陕哲罗鲑精子类型较为原始，属于硬骨鱼类中的TypeⅠ类型。<br>英文摘要：This study was conducted to investigate the morphology and ultrastructure of Hucho bleekeri Kimura spermatozoa through scanning and transmission electron microscopy. The results revealed that the spermatozoa can be differentiated into three major parts: a head, a mid-piece, and a flagellum. The mean length of the spermatozoa was 41.07 μm±2.18 μm, with a spherical head length of 2.76 μm±0.15 μm and a flagellum length of 34.74 μm±5.01 μm. The mean anterior and posterior head widths were 1.88 μm±0.18 μm and 2.08 μm±0.20 μm, respectively. The head was spherical without acrosome, which was composed of nucleus. The short mid-piece was located laterally to the nucleus and possessed just one spherical mitochondrion with a mean diameter of 0.82 μm±0.08 μm. The spermatozoa flagellum was long and cylindrical, and could be separated into two parts. The axoneme composed the typical "9+2" microtubular doublet structure and was enclosed by the cell membrane. The flagellum had short irregular side-fins. This study confirmed that H. bleekeri Kimura spermatozoa can be categorized as teleostean Type Ⅰ; "primitive" or "ect-aquasperm type" spermatozoa. 2016,35(4): 550-555 收稿日期：2016-03-07 DOI：10.11984/j.issn.1000-7083.20160047 分类号：Q959.4 基金项目：公益性行业（农业）科研专项（201203086）；国家自然科学基金青年项目（31402301）；中国水产科学研究院基本科研业务费（2014A07XK05） 作者简介：郭威(1989-),男,硕士研究生,研究方向为濒危鱼类保护,E-mail:guowei612@escience.cn *通讯作者：危起伟,E-mail:weiqw@yfi.ac.cn 参考文献： 丁瑞华, 桂林华, 李明, 等. 2010. 虎嘉鱼及其保护生物学[J]. 广西师范大学学报(自然科学版), 28(2):96-102. 董崇智, 李怀明, 赵春刚, 等. 1998. 濒危名贵哲罗鱼保护生物学的研究Ⅰ.哲罗鱼分布区域及其变化[J]. 水产学杂志, 11(5):65-70. 杜浩, 李罗新, 危起伟, 等. 2014. 濒危物种川陕哲罗鲑在汉江上游太白河再发现[J]. 动物学杂志, 49:414. 谷伟, 张永泉, 户国, 等. 2014.虹鳟精子的扫描和透射电镜观察[J]. 水产学杂志, (5):24-27. 刘长琳, 庄志猛, 陈四清, 等. 2009. 星突江鲽精子的超微结构[J]. 渔业科学进展, (4):14-20. 申志新, 唐文家, 李柯懋. 2005. 川陕哲罗鲑的生存危机与保护对策[J]. 淡水渔业, 35(4):25-28. 杨德国, 危起伟, 李绪兴, 等. 1999. 秦岭湑水河太白段珍稀水生动物分布现状及保护对策[J]. 中国水产科学, 6(3):124-126. 张永泉, 尹家胜, 刘奕, 等. 2011. 白斑红点鲑精子形态和超微结构的研究[J]. 上海海洋大学学报, (6):820-825. Afzelius B. 1978. Fine structure of the grafish spermatozoa[J]. Journal of Ultrastruct Research, 64:309-314. Baccetti B, Burrini AG, Callaini G, et al. 1984. Fish germinal cells:comparative spermatology of 7 cyprinid species[J]. Gamete Research, 10:373-396. Billard R, Cosson J, Linhart O. %K 川陕哲罗鲑 %K 精子 %K 超微结构< %K br> %K 英文关键字： Hucho bleekeri Kimura %K spermatozoa %K ultrastructure %U http://www.scdwzz.com/viewmulu.aspx?qi_id=1584&mid=49381&xuhao=11