%0 Journal Article %T 长足大竹象雄性附腺提取物抑菌作用研究<br>Study on Antibacterial Activity of Male Accessory-gland Extracts from Cyrtotrachelus buqueti %A 梁梓 %A 杜超豪 %A 杨瑶君 %A 农向 %A 廖鸿 %A 颜珊 %J 四川动物 %D 2016 %X 中文摘要：以3种细菌(大肠杆菌 Escherichia coli、金黄色葡萄球菌 Bacillus subtilis、枯草芽孢杆菌 Staphyloccocus aureus)为供试菌,测定了长足大竹象 Cyrtotrachelus buqueti雄性附腺提取物的抑菌活性。结果表明:长足大竹象雄性附腺提取物对革兰氏阳性菌如枯草芽孢杆菌、金黄色葡萄球菌均有一定的抑菌活性。不同浓度的粗提物对枯草芽孢杆菌及金黄色葡萄球菌抑菌影响差异均具有高度统计学意义( P<0.01),抑菌活性随着粗提物浓度的增加而增强。粗提物对金黄色葡萄球菌和枯草芽孢杆菌的最小抑菌浓度分别为0.2 mg·mL -1、0.4 mg·mL -1。不同处理温度对长足大竹象雄性附腺粗提物的抑菌作用有一定影响。<br>英文摘要：The antibacterial activities of male accessory-gland extracts from Cyrtotrachelus buqueti were determined using three species of bacteria ( Escherichia coli, Bacillus subtilis, and Staphyloccocus aureus). The results showed that male accessory-gland extracts from C. buqueti had a certain antibacterial activity on the Gram-positive bacteria such as B. subtilis and S. aureus, and the antibacterial activities were significantly differed among different concentrations of male accessory-gland extracts. The antibacterial activity would increase with increasing concentrations of the extracts. The minimum inhibitory concentration of male accessory-gland extracts against B. subtilis and S. aureus were 0.2 mg·mL -1 and 0.4 mg·mL -1, respectively. Temperature also had a certain influence on the antibacterial effects of male accessory-gland extracts from C. buqueti. 2016,(1): 66-69 收稿日期：2015-05-01 DOI：10.11984/j.issn.1000-7083.20150234 分类号：Q965 基金项目：四川省教育厅项目(13ZB0112) 作者简介：梁梓(1980-), 女, 高级实验师, 研究方向: 生物化学, E-mail:120564533@qq.com *通讯作者：杨瑶君, 博士, 教授, 研究方向: 林竹生态, E-mail:290316289@qq.com 参考文献： 高洁, 嵇保中, 刘曙雯, 等. 2008. 桑天牛雄性附腺内容物组分分析[J]. 四川林业科技, 29(6): 58-62. 高熹, 吴国星, 叶恭银, 等. 2007. 棕尾别麻蝇雄性附腺分泌物的生理功能[J]. 昆虫知识, 44(6): 821-825. 向玉勇, 周书芹, 程凯, 等. 2013. 家蝇幼虫血淋巴粗提物抑菌活性初步研究[J]. 四川动物, 32(3): 419-422. 沈萍, 范秀容, 李广武, 等. 1999. 微生物学实验[M]. 北京: 高等教育出版社: 215. 孙恩涛, 秦志辉. 2006. 昆虫抗菌肽研究进展[J]. 热带病与寄生虫学, 4(1): 47-50. 王维德, 王雄清, 陈封政. 2002. 沐川县竹林主要害虫大竹象的调查[J]. 乐山师范学院学报, 19(4): 49-50. 温旺荣, 戴庚孙, 王德春. 1994. 常用消毒剂对念珠菌最小杀菌浓度的测定[J]. 中国消毒学杂志, 11(2): 85-87. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 72: 248-254. Chapman T, Davies SJ. 2004. Functions and analysis of the seminal fluid proteins of male Drosophila melanogaster fruit flies[J]. Peptides, 25(9): 147-190. Lung O, Wolfner MF. 1999. Drosophila seminal fluid proteins enter the circulatory system through the walls of the posterior vagina[J]. Insect Biochemistry and Molecular Biology, 29: 1043-1052. Lung O, Wolfner MF. 2001. Identification and characterization of the major Drosophila melanogaster mating plug protein[J]. Insect Biochemistry and Molecular Biology, 31: %K 长足大竹象 %K 雄性附腺 %K 抑菌作用< %K br> %K 英文关键字： Cyrtotrachelus buqueti %K male accessory glands %K antibacterial activity %U http://www.scdwzz.com/viewmulu.aspx?qi_id=1524&mid=47698&xuhao=11