%0 Journal Article
%T 抗生素保存褐马鸡粪便分泌型免疫球蛋白A的时效性研究<br>Effects of Antibiotics on the Storage of Fecal Secretory Immunoglobulin A of Brown-eared Pheasant
%A 周冉
%A 齐磊
%A 王文霞
%A 胡德夫
%A 隋金玲
%J 四川动物
%D 2016
%X 中文摘要：粪便免疫球蛋白是衡量野生动物健康状况及生存状态的有效手段之一,其中粪便的保存问题是关键。为探索抗生素对粪便分泌型免疫球蛋白A(SIgA)的保存时效性,采用酶联免疫吸附法对4种常用抗生素(利福平、四环素、卡那霉素和庆大霉素)的3种不同浓度(1 μg·mL -1、25 μg·mL -1、50 μg·mL -1)保存的褐马鸡 Crossoptilon mantchuricum粪便SIgA的含量进行了测定。结果表明,25 μg·mL -1的利福平保存的褐马鸡粪便SIgA效果最佳,其次是50 μg·mL -1的四环素和50 μg·mL -1的卡那霉素,庆大霉素保存效果最差。<br>英文摘要：Evaluation of fecal immunoglobulin level is an effective means of monitoring health and survival situation in wildlife, and this method requires the storage of feces in an appropriate way. In the present study, four antibiotics including rifampicin, tetracycline, kanamycin and gentamicin were used to investigate the efficiency for preservation of fecal secretory immunoglobulin A (SIgA) of brown-eared pheasant (Crossoptilon mantchuricum) at three different concentrations (1 μg·mL -1, 25 μg·mL -1, 50 μg·mL -1). The content of SIgA was measured by enzyme linked immunosorbent assay. The results showed that rifampicin at concentration of 25 μg·mL -1 was optimal for SIgA preservation, followed by 50 μg·mL -1 tetracycline and 50 μg·mL -1 kanamycin, whereas the storage effect of gentamicin was the worst. 2016,(1): 58-61 收稿日期：2015-05-22 DOI：10.11984/j.issn.1000-7083.20150190 分类号：Q955 基金项目：全国第二次陆生野生动物资源调查--北京市常规调查项目(YBZ2015-003) 作者简介：周冉(1989-), 女, 硕士研究生, 研究方向为野生动物保护与利用, E-mail:zhou_ran@126.com *通讯作者：隋金玲, 副教授, E-mail:jlsui@126.com 参考文献： 郎冬梅, 刘文华, 胡德夫, 等. 2011. 圈养林麝粪便类固醇激素保存时效性研究[J]. 四川动物, 30(3): 357-361. 雷忻, 廉振民. 2008. 陕西黄龙山褐马鸡生存现状研究[J]. 科技导报, 26(14): 58-60. 李青, 张紫媛, 樊均明. 2010. 各种细菌产生的SIgA蛋白酶的结构、性质及作用[J]. 中国中西医结合肾病杂志, (8): 740-742. 柳明来, 党太合. 1999. 褐马鸡的生态习性及其保护意义[J]. 陕西林业科技, 3: 49-50. 武玉珍, 冯睿芝. 2013. 褐马鸡的濒危原因及保护措施[J]. 中国家禽, 35(8): 49-50. 张国刚, 郑光美, 张正旺. 2005. 栖息地特征对褐马鸡种群密度和集群行为的影响[J]. 生物多样性, 25(13): 162-167. 郑光美, 王歧山. 1998. 中国濒危动物红皮书(鸟类)[M]. 北京: 科学出版社: 242-243. 郑建旭, 安春林, 郭书彬. 2000. 小五台山自然保护区珍禽褐马鸡繁殖生物学习性研究[J]. 河北林业科技, (4): 5-7. Currya E, Stoopsa MA, Rotha TL. 2012. Non-invasive detection of candidate pregnancy protein biomarkers in the feces of captive polar bears ( Ursus maritimus)[J]. Theriogenology, 78(2): 308-314. Khan MZ, Altmann J, Isani SS, et al. 2002. A matter of time: evaluating the storage of fecal samples for steroid analysis[J]. General and Comparative Endocrinology, 128(1): 57-64. Paramastri Y, Royo F, Eberova J, et al. 2007. Urinary and fecal immunoglobulin A, cortisol and 11-17 dioxoandrostanes, and serum cortisol in metabolic cage housed female cynomolgus monkeys ( Macaca fascicularis)[J]. Journal of Medical Primatology, 36(6): 355-364. Peters I, Calvert E, Hall E, et al. 2004. Measurement of immunoglobulin concentrations in the feces of healthy
%K 褐马鸡
%K 抗生素
%K 粪便分泌型免疫球蛋白A
%K 保存时效&lt
%K br&gt
%K 英文关键字： Crossoptilon mantchuricum
%K antibiotics
%K fecal secretory immunoglobulin A
%K storage time
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