%0 Journal Article
%T 磷氧氮丙啶不育剂对雄性红耳龟生殖毒性研究<br>Reproductive Toxicity Research of MAPO on Male Trachemys scripta elegans
%A 龚声辉
%A 傅丽容
%A 张海会
%A 张瑞红
%A 刘明娜
%A 史海涛
%J 四川动物
%D 2015
%X 中文摘要： 目的 研究磷氧氮丙啶(MAPO)对红耳龟 Trachemys scripta elegans睾丸形态学及组织病理学的影响,从控制繁殖的途径初步探讨外来物种红耳龟的防治。 方法 选取160只健康成体雄性红耳龟,随机平均分为4组,设置MAPO相应浓度梯度,对照组和3个试验组(0.1 g·L -1、3 g·L -1、10 g·L -1),腹腔注射15 d。 结果 低、中剂量组平均摄食量与对照组相比变化不明显( P>0.05),高剂量组平均摄食量、睾丸体积及脏器系数显著减小( P<0.05);组织病理学观察显示,各处理组睾丸均有不同程度的损伤,中、高剂量组曲细精管萎缩,各级生精细胞几乎全部退化消失,生精上皮厚度极显著低于对照组( P<0.01);MAPO对血清和睾丸中睾酮含量无影响。 结论 MAPO可通过作用各级生精细胞的发育,导致不育,可进一步开发为红耳龟不育防控药物,应用于生产实践。<br>英文摘要： Objective To investigate the testicular morphology and histopathology effect of MAPO on male Trachemys scripta elegans, we preliminarily investigated the way to prevent T. scripta elegans by breeding control. Methods A total of 160 adult health male T. scripta elegans were randomly divided into three experiment groups and one control group. The turtles in three experiment groups were treated with normal saline at the concentration of 0.1 g·L -1, 3 g·L -1, 10 g·L -1 MAPO by intraperitoneal injection for 15 days, respectively; the turtles in control group were treated with normal saline for 15 days. Results Compared with the control group, the food consumption of low-dose group and middle-dose group were not significantly different ( P>0.05); the volume and organ coefficient of testis and the food intake in high-does group declined very obviously ( P<0.01). According to pathologic histological observation, the testis of all the MAPO treated male turtles showed a certain degree of damage, and the seminiferous of the middle-does group and high-does group were atrophied. The majority of germ cells were necrotic and the thickness of seminiferous epithelium diminished very significantly compared with the control group ( P<0.01). MAPO had no effect on testosterone in serum and testis. Conclusions MAPO can cause dysgenesis of T. scripta elegans by blocking the development of germ cell and can be used to control its population size. 2015,(): 677-682 收稿日期：2015-3-4 DOI：10.11984/j.issn.1000-7083.20150072 分类号：Q959.6 基金项目：国家自然科学基金资助项目(31372228);海南省自然科学基金资助项目(314078) 作者简介：龚声辉(1989-),男,硕士研究生,研究方向:动物生态学及保护生物学,E-mail:15120755727@163.com *通讯作者：史海涛,E-mail:haitao-shi@263.net 参考文献： 陈贤均, 赵红刚. 2005. 盐制杜仲对小鼠生长发育与脏器系数的影响[J]. 四川中医, 23(11):29-31. 李闯. 2013. 海南万泉河红耳龟( Trachemys scripta elegans)野外繁殖研究[D]. 海南:海南师范大学. 廖明阳, 马华智, 王治乔, 等. 1998. 磷氧氮丙啶诱发CHO细胞HGPRT基因位点正向突变的分子特征[J]. 中国公共卫生学报, 17(3):137-138. 刘丹, 史海涛, 刘宇翔, 等. 2011. 红耳龟在我国分布现状的调查[J]. 生物学通报, 46(6):18-21. 刘巍. 2006. M001雄性不育灭鼠剂的生殖毒性研究及其在农业鼠害防治方面的应用[D]. 北京:中国协和医科大学. 史海涛, 龚世平, 梁伟, 等. 2009. 控制外来物种红耳龟在中国野生环境蔓延的态势[J]. 生物学通报, 44(4):1-4. 田永路, 于洪江, 张希牧, 等. 2009. 5~7周龄SD和Wistar大鼠主要脏器系数及体尺的测定[J]. 实验动物科学,
%K 磷氧氮丙啶
%K 红耳龟
%K 不育防治&lt
%K br&gt
%K 英文关键字：MAPO
%K Trachemys scripta elegans
%K contraception control
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