%0 Journal Article
%T 基质金属蛋白酶-9及金属蛋白酶组织抑制剂-1在孕鼠胎盘形成中期的表达模式<br>The Expression Pattern of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Placentation of Pregnant Mice
%A 魏孟梅
%A 吴曙光
%A 赵海
%J 四川动物
%D 2016
%X 中文摘要： 目的 探讨基质金属蛋白酶-9(MMP-9)及金属蛋白酶组织抑制剂-1(TIMP-1)在孕鼠胎盘形成中期的表达模式。 方法 HE染色观察孕鼠第9天至第14天(D9~D14)胎盘形态结构的变化,同时免疫组织化学法检测相应天数胎盘中MMP-9和TIMP-1的表达情况。 结果 HE染色结果显示孕鼠D11时胎盘的3层结构开始形成,包括蜕膜区(Dec)、海绵滋养细胞层(Sp)和迷路区(Lab)；随着妊娠天数的增加,Dec区域逐渐缩小,Sp和Lab区域增大；免疫组化表明,MMP-9和TIMP-1均表达于细胞浆和细胞核中,MMP-9强表达于D9和D10胎盘的外胎盘锥中,D10~D13的Dec、Sp和D11~D13的滋养巨细胞中。MMP-9在D11的Lab,D12的Dec和Lab,D13的Dec、Sp、Lab区域中的阳性细胞的积分光密度值与D14胎盘中对应值比较,差异有统计学意义( P<0.05)；TIMP-1在D9~D12的Dec区域中的阳性细胞的积分光密度值与D14胎盘中对应值比较,差异有统计学意义( P<0.05)；MMP-9/TIMP-1在D9、D11的Dec及D12的Lab区域中的比值与D14胎盘中对应值比较,差异有统计学意义( P<0.05),在D10、D12的Dec和D13的Lab区域中的比值与D14胎盘中对应值比较,差异有高度统计学意义( P<0.01)。 结论 MMP-9和TIMP-1在孕鼠胎盘中的协同表达可能参与调节胎盘的形成。<br>英文摘要： Objective To study the expression pattern of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in placentation of pregnant mice. Methods HE staining was used to check the morphological structure of placentas during pregnancy (day 9~day 14). Besides, the expression levels of MMP-9 and TIMP-1 in placentation were detected by using immunohistochemistry. Result The morphological structure of placenta began to form on day 11, which was constituted of decidua basalis (Dec), spongiotrophoblast (Sp), and labyrinth (Lab) of pregnant mice; the area of Dec was decreased, whereas the area of Sp and Lab were increased with the increasing of pregnancy days; the expression of MMP-9 and TIMP-1 were mainly distributed in cytoplasm and cell nucleus, which was strong expressed in ecto-placental cone of placenta on day 9 and day 10, the Dec, Sp from day 10 to 13 and trophoblast giant cells from day 11 to 13. The integrated optical density (IOD) of MMP-9 in Lab of day 11, in Dec and Lab of day 12, and in placenta of day 13 were significantly higher than that of day 14 ( P<0.05); IOD of TIMP-1 in Dec during day 9 to 12 was significantly lower than that of day 14 ( P<0.05); the ratio of MMP-9/TIMP-1 in Dec on days 9 and 11 and in Lab on day 12 were significantly higher than that of day 14 ( P<0.05); the ratio of MMP-9/TIMP-1 in Dec on days 10 and 12 and in Lab on day 13 were also very significantly higher than that of day 14 ( P<0.01). Conclusion MMP-9 and TIMP-1 may play an important role in regulating the formation of the placenta by collaborative expression. 2016,35(5): 703-708 收稿日期：2016-04-03 DOI：10.11984/j.issn.1000-7083.20160072 分类号：Q954 作者简介：魏孟梅,硕士研究生,从事药物毒理研究,E-mail:1432016508@qq.com *通讯作者：赵海,硕士,助理实验师,从事胎盘形成研究,E-mail:344947218@qq.com 参考文献： 常雅丽, 田瑛, 赵志茹, 等. 2014. CD147与MMP-2在葡萄胎组织中的表达及其意义[J]. 中国妇幼保健, 29(3): 431-434. 陈春莹, 李丽, 黄海玉, 等. 2012. Ets-1和MMP-9在胎膜早破中的表达和意义[J]. 中国妇幼保健,
%K 金属蛋白酶
%K 金属蛋白酶组织抑制剂
%K 胎盘
%K 外胎盘锥&lt
%K br&gt
%K 英文关键字：matrix metalloproteinases
%K tissue inhibitor of metalloproteinases
%K placenta
%K ectoplacental cone
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