%0 Journal Article
%T 绿翅短脚鹎线粒体基因组测序分析<br>Sequencing and Analysis of the Mitochondrial Genome of Ixos mcclellandii
%A 陈映竹
%A 宋旭颢
%A 周闯
%A 张文博
%A 陈本平
%A 吴少彬
%A 岳碧松
%J 四川动物
%D 2018
%R 10.11984/j.issn.1000-7083.20180121
%X 中文摘要：本文对绿翅短脚鹎 Ixos mcclellandii线粒体基因组进行了测序分析。结果显示，绿翅短脚鹎线粒体基因组序列全长17 838 bp（GenBank登录号：KX640824），具有2个开放阅读框重叠区，即ATP6~ATP8（10 bp）和ND4L~ND4（7 bp），还有一些重叠发生在蛋白质基因和其相邻的tRNA基因之间。除COX Ⅰ、ND3的起始密码子分别为GTG、ATA外，其余11个蛋白质基因的起始密码子均为ATG。9个蛋白质基因以TAA、AGA或AGG为终止密码子，其余则以T（COX Ⅲ和ND4）或TA（ND2和ND4L）为终止密码子。蛋白质基因使用频率最高的密码子是CUA（217次）和ACC（205次），而GGU和GCG的使用频率最低，均为24次。tRNA基因分布在rRNA基因和蛋白质基因之间，长度为64~75 bp。通过分析已报道的雀形目Passeriformes鸟类线粒体控制区结构，发现了3种不同类型的控制区结构：1）仅存在1个控制区；2）有2个长度相近且序列高度相似的控制区；3）有2个高度异质的控制区。绿翅短脚鹎线粒体基因组含有2个高度相似的控制区（相似度91.6%），长度分别为1 116 bp和1 144 bp，二者仅在控制区开始和末端部位的序列有所不同。<br>英文摘要：In this study, the complete mitochondrial genome of Ixos mcclellandii was determined. This circular genome of I. mcclellandii was 17 838 bp in length (GenBank accession: KX640824). The mitogenome harbored 2 overlapping protein regions, i.e., ATP6-ATP8 (10 bp) and ND4L-ND4 (7 bp), and there were also some overlapping regions positioned between the protein genes and their flanking tRNA genes. Conventional stop codons (i.e., TAA, AGA or AGG) could be assigned to most of the protein coding genes, while the rest ended with an incomplete stop codon T (COX Ⅲ and ND4) or TA (ND2 and ND4L). Among the 13 protein-coding genes, the most frequently used codons were CUA and ACC (217 times and 205 times, respectively), while the most infrequently used codons were GGU and GCG (only 24 times). The length of tRNA ranges from 64 bp to 75 bp, which loacated between rRNA genes and protein coding genes. Based on the new data and the publicly accessible mitochondrial genomes in Passeriformes, 3 types of mitochondrial control regions were found: 1) Only one control region; 2) Two control regions with high similarity (control region 1+control region 2); 3) Two highly heterogeneous control regions (control region+non-coding region). The mitochondrial genome of I. mcclellandii contained 2 control regions, which were determined to be 1 116 bp and 1 144 bp in length, respectively. Furthermore, the intra-genomic similarity between the 2 noncoding regions was detected (91.6%). 2018,37(6): 646-652 收稿日期：2018-04-16 分类号：Q959.7;Q731 基金项目：重庆市自然科学基金项目（cstc2011jjA80035） 作者简介：陈映竹(1992—),女,硕士研究生,研究方向:生态学,E-mail:497615422@qq.com *通信作者：岳碧松,E-mail:bsyue@scu.edu.cn 参考文献： 白洁. 2012.大山雀和绿背山雀线粒体基因组序列测定和系统发生分析[D]. 西安: 陕西师范大学. 陈娟. 2015. 鹰形目和雀形目七种鸟类的线粒体全基因组序列的测序与分析[D]. 芜湖: 安徽师范大学. 陈四海, 区又君, 李加儿. 2011. 鱼类线粒体DNA及其研究进展[J]. 生物技术通报, 3: 13-20. 邓青珊, 朱琼琼, 鲁长虎. 2008. 南方红豆杉的天然更新格局及食果鸟类对其种子的传播[J]. 生态学杂志, 27: 712-717. 丁虎林. 2013. 浙江天童三种鹎科鸟类栖息地选择的季节变化[D]. 上海: 华东师范大学. 高瑞瑞. 2013. 三种雀形目鸟类线粒体基因组测定及雀形目鸟类系统发生研究[D]. 西安: 陕西师范大学. 林立亮. 2011.
%K 雀形目
%K 绿翅短脚鹎
%K 线粒体基因组
%K 控制区&lt
%K br&gt
%K 英文关键字：Passeriformes
%K Ixos mcclellandii
%K mitochondrial genome
%K control region
%U http://www.scdwzz.com/viewmulu.aspx?qi_id=1870&mid=58197&xuhao=6