%0 Journal Article
%T 富血小板纤维蛋白超微结构观察及意义
%A 陈铁楼
%A 江一峰
%A 张新海
%A 陈骏
%A 黄传梅
%A 王晓熙
%J 同济大学学报(医学版)
%D 2018
%R 10.16118/j.1008-0392.2018.04.009
%X 目的 探讨富血小板纤维蛋白（platelet rich fibrin, PRF）形态学超微结构及意义。方法 选择10例健康志愿者，抽取静脉血制备PRF，对PRF分别行光学显微镜、扫描电子显微镜（scanning electron microscope, SEM）和透射电子显微镜（transmission electron microscope, TEM）观察。结果 PRF为淡黄色纤维蛋白凝胶，H-E染色见胶原纤维为染色均一的淡红色网状结构，在近细胞端纤维排列较致密，含大量血小板。吉姆萨染色见淡蓝色的胶原纤维为网状结构，近细胞端见染为蓝色的白细胞。SEM发现胶原纤维排列成疏松多孔网状支架结构，含大量静止或伸出伪足的血小板及白细胞。TEM发现血小板处于静止或激活状态，α-颗粒释放于细胞和纤维之间，在细胞之间见高密度区为血小板释放的颗粒。结论 PRF胶原纤维的网状立体结构和富含血小板激活释放生长因子，对生物学作用发挥有重要意义。</br>Objective To investigate the morphological ultrastructure of platelet rich fibrin（PRF）and its implication. Methods PRF was prepared from venous blood samples from 10 healthy donors. The PRF was observed by light microscopy (H-E staining and Wright Giemsa staining), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. Results PRF presented as light yellow fibrin gel. HE staining showed that collagen fiber was of uniform and light red network structure. In the near cell end, the fibers were denser and contained lots of platelets. Giemsa staining showed that the collagen fibers were of light blue mesh structure, and the dark blue white blood cells were in the near cell end. SEM observation found that collagen fibers were arranged in a porous reticular scaffold, containing a large number of static or protruding platelets and leukocytes. TEM showed that platelets were at rest or activated, α-particles were released between platelets or fibers, which displayed a high density area between cells. Conclusion Through the reticular stereoscopic structure of collagen fibers and rich platelets activating growth factor release, PRF plays an important biological role
%K 富血小板纤维蛋白 形态学 超微结构 扫描电镜 透射电镜&lt
%K /br&gt
%K platelet rich fibrin morphology ultrastructure scanning electron microscope transmission electron microscope
%U http://tjyxxb.cnjournals.cn/ch/reader/view_abstract.aspx?file_no=20180409&flag=1