%0 Journal Article
%T 骨髓间充质干细胞培养液对牙周组织再生的影响
%A 倪璞
%A 刘宏伟
%J 同济大学学报(医学版)
%D 2018
%R 10.16118/j.1008-0392.2018.01.009
%X 目的 观察大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells, BMMSCs）培养液对牙周组织再生的影响。方法 原代培养SD大鼠BMMSCs，取细胞培养液（conditioned media, CM）。采用ELISA检测培养液里与成骨相关的IGF-1、VEGF、PDGF-BB、BMP-2的含量；利用BMMSCs培养液对牙周膜细胞（periodontal ligament cells, PDLCs）进行培养后，RT-PCR法检测PDLCs矿化相关基因核心结合因子2（Runx2）、骨桥素（OPN）、骨钙素（OCN）的变化；Western印迹法检测Runx2、OPN、OCN蛋白的表达变化。将BMMSCs培养液及种子细胞分别制备成凝胶，在大鼠左下第一磨牙颊侧及根分叉处制备牙周缺损，放入制备好的凝胶。分别在术后第4、8周，处死大鼠，取出左侧下颌骨，进行Micro-CT扫描及组织学分析。结果 BMMSCs培养液中含有大量IGF-1和VEGF；RT-PCR和Western印迹法均能检测到牙周膜细胞OPN、OCN、Runx2的表达，且利用BMMSCs培养液培养的PDLCs表达高于常规培养的PDLCs(P＜0.05)。8周时，BMMSCs培养液凝胶组相比于对照组生成了更多的牙槽骨(P＜0.05)。结论 同等条件下,BMMSCs培养液比种子细胞能更好地修复缺失的牙周组织。</br>Objective To investigate the effects of conditioned media(CM) of rat bone marrow mesenchymal stem cells (BMMSCs) on periodontal tissue regeneration. Methods Primary bone marrow stromal stem cells were isolated and cultured in vitro. The IGF-1, VEGF, PDGF-BB and BMP-2 levels in conditioned media of BMMSCs were measured by ELISA. The periodontal ligament cells (PDLCs) were cultured with conditioned media, the expression of Runx2, OCN OPN mRNA and protein was detected by RT-PCR and Western blotting, respectively．The gels containing BMMSCs (control group) or their conditioned (test group) were implanted in prepared periodontal defects of rat mandible. After 4 and 8 weeks, the implant sections were examined by micro-CT and histological analysis. Results The conditioned media contains large amounts of IGF-1 and VEGF. The expression levels of Runx2, OPN, OCN mRNAs and protein were up-regulated (P＜0.05)．After 8 weeks, more bones were recovered in the conditioned media group compared with the control group (P＜0.05). Conclusion Conditioned media of BMMSCs can repair the periodontal defect better than seed cells
%K 牙周组织再生 牙周缺损 骨髓间充质干细胞 牙周膜细胞 培养液&lt
%K /br&gt
%K periodontal tissue regeneration periodontal defect bone marrow mesenchymal stem cells periodontal ligament cells conditioned media
%U http://tjyxxb.cnjournals.cn/ch/reader/view_abstract.aspx?file_no=20180109&flag=1