%0 Journal Article %T 苦参碱通过P38通路调节H2AX磷酸化抑制宫颈癌细胞的增殖及迁移 %A 赵淑婷 %A 雷蕾 %A 程静新 %J 同济大学学报(医学版) %D 2018 %R 10.16118/j.1008-0392.2018.04.005 %X 目的 探讨苦参碱对宫颈癌细胞增殖、凋亡、迁移的影响及其可能的分子机制。方法 不同浓度苦参碱作用于宫颈癌细胞，通过CCK8法，EdU法、流式细胞术、Transwell实验、划痕实验检测细胞的增殖、凋亡、迁移能力。利用Western印迹法检测苦参碱对H2AX磷酸化蛋白（即γH2AX）、P38磷酸化蛋白（即pP38）水平的影响。通过干扰H2AX（Ser139）位点的磷酸化，检测苦参碱对宫颈癌细胞增殖、迁移的影响。通过干扰P38磷酸化，检测苦参碱对H2AX磷酸化的影响。结果 苦参碱对宫颈癌细胞体外增殖具有显著抑制作用（P<0.05），且呈药物浓度依赖性；苦参碱显著提高细胞凋亡率，而对迁移能力具有显著抑制作用（P<0.05）；苦参碱能够显著促进γH2AX及pP38蛋白的表达（P<0.05），且呈浓度依赖性。H2AX（Ser139）位点的磷酸化是苦参碱发挥抑增殖和迁移的作用位点。苦参碱通过P38通路调控H2AX（Ser139）位点的磷酸化。结论 苦参碱抑制宫颈癌细胞的增殖和迁移，诱导细胞凋亡，可能是通过调控P38通路使H2AX（Ser139）位点磷酸化发挥对宫颈癌的抑癌作用。</br>Objective To investigate the effects of matrine on the proliferation and migration of cervical cancer cells and the underlying mechanisms. Methods CCK-8 assay, EdU assay, Transwell assay and wound-healing assay were applied to examine the proliferation, apoptosis and migration of cervical cancer SiHa and C33A cells. Western blotting was applied to detect the expression of H2AX and P38 in cervical cancer cells. CCK8 and Transwell assay were used to detect the effect of matrine on the cell proliferation and migration by interfering with H2AX phosphorylation. Western blot was used to detect the H2AX phosphorylation by interfering with P38 phosphorylation. Results Matrine suppressed the proliferation（P<0.05）, induced apoptosis and inhibited the migration of cervical cancer cells（P<0.05）. Further investigation discovered that matrine significantly induced the phosphorylation of the H2AX and P38 in a dose-dependent manner（P<0.05）. Matrine inhibited proliferation and migration by phosphorylation of H2AX (Ser139) site. Matrine regulated phosphorylation of H2AX (Ser139) site through the P38 pathway. Conclusion Matrine can inhibit cervical cancer cell proliferation and migration in vitro and this action may be related to P38 signaling pathway and phosphorylation of H2AX (Ser139) site %K 苦参碱 宫颈癌 增殖 P38 H2AX基因< %K /br> %K matrine cervical cancer proliferation P38 H2AX %U http://tjyxxb.cnjournals.cn/ch/reader/view_abstract.aspx?file_no=20180405&flag=1