%0 Journal Article
%T RNA/DNA Ratio and LPL and MyoD mRNA Expressions in Muscle of Oreochromis niloticus Fed with Elevated Levels of Palm Oil RNA/DNA Ratio and LPL and MyoD mRNA Expressions in Muscle of Oreochromis niloticus Fed with Elevated Levels of Palm Oil
%A Christian Larbi AYISI
%A ZHAO Jinliang
%J 中国海洋大学学报（英文版）
%D 2016
%X Palm oil is of great potential as one of the sustainable alternatives to fish oil(FO) in aquafeeds.In this present study,five isonitrogenous diets(32% crude protein) with elevated palm oil levels of 0%,2%,4%,6% and 8% were used during an 8-week feeding trial to evaluate its effects on RNA/DNA ratio and lipoprotein lipase(LPL) and Myo D m RNA expressions in muscle of Oreochromis niloticus.The results showed that RNA,DNA content as well as ratio of RNA to DNA were significantly affected(P < 0.05),in each case the highest was recorded in fish group subjected to 6% palm oil level.There was a strong positive correlation between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and specific growth rate(SGR),protein efficiency ratio(PER),while a negative correlation existed between nucleic acid concentration(RNA concentration and RNA:DNA ratio) and feed conversion ratio(FCR).The m RNA expressions of LPL and Myo D in muscle were not significantly affected by the different palm oil levels,although the highest expression was observed in fish fed with 6% palm oil level.There also existed a strong positive correlation between the m RNA expression of LPL,Myo D and SGR,PER,while their correlation with FCR was negative.In conclusion,elevated palm oil affected the RNA,DNA concentration as well as RNA/DNA ratio significantly,although the m RNA expression of LPL and Myo D were not affected significantly by elevated palm oil levels
%U http://qdhb.cbpt.cnki.net/WKD/WebPublication/paperDigest.aspx?paperID=12e0fb52-9bdf-46bc-a978-a8d06421d032