%0 Journal Article
%T Development of a Fast ELISA for the Specific Detection of both Leucomalachite Green and Malachite Green Development of a Fast ELISA for the Specific Detection of both Leucomalachite Green and Malachite Green
%A JIANG Yousheng
%A CHEN Li
%A HU Kun
%A YU Wenjuan
%A YANG Xianle
%A LU Liqun
%J 中国海洋大学学报（英文版）
%D 2015
%X Malachite green(MG), a dye, is an antifungal agent that has been used to treat and prevent fish diseases. It is metabolized into reduced leucomalachite green forms(LMG) that may reside in fish muscles for a long period, thus being harmful to human health. The aim of this study was to develop a competitive and direct enzyme-linked immunosorbent assay(ELISA) to detect MG and LMG specifically. The monoclonal antibody(m Ab) to LMG was generated using a hybridoma technique. The obtained m Ab showed good cross-reactivity(CR) to malachite green(MG), but not to crystal violet(CV) and Brilliant Green(BG). The m Ab was used to develop a fast detecting ELISA of MG and LMG in fish. By introducing the conjugation LMG-HRP, the detection capability was 0.37 ng m L-1 for MG and LMG. The mean recovery from spiked grass carp tissues ranged from 76.2% to 82.9% and the coefficients of variation varied between 1.8% and 7.5%. The stable and efficient monoclonal cell line obtained is a sustainable source of sensitive and specific antibody to MG and LMG
%U http://qdhb.cbpt.cnki.net/WKD/WebPublication/paperDigest.aspx?paperID=08e10ab7-12a1-4ec2-92f8-1d1c9883b4a3