%0 Journal Article
%T 全身低能量光疗对重度烧伤大鼠早期全身炎症反应的影响
%A 金剑
%A 何放
%A 罗鹏飞
%A 胡晓燕
%A 夏照帆
%J 第二军医大学学报
%D 2017
%R 10.16781/j.0258-879x.2017.08.0987
%X 目的 探讨全身低能量光疗（LLLT）对重度烧伤大鼠全身炎症反应的影响。方法 将50只SD大鼠随机分为空白对照组、单纯烧伤组、烧伤后单次短时光照组、烧伤后单次长时光照组和烧伤后多次短时光照组，每组10只。单次短时光照组、烧伤后单次长时光照组、烧伤后多次短时光照组大鼠在烧伤后均给予低强度LED光（640 nm）照射全身，分别照射5 min/d、15 min/d、5 min×3次/d。各组大鼠分别于造模前1 d，造模后即刻，造模后第1、3、7、14天取尾静脉血检测血清肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1β、IL-6的含量，计数静脉血白细胞；并于造模后第3、7、14天测量创面面积，计算创面愈合率。结果 与空白对照组相比，造模后第1天时单纯烧伤组及烧伤后单次短时光照组大鼠血清TNF-α含量升高（P<0.05），而烧伤后单次长时光照组及烧伤后多次短时光照组分别于造模后第1、7天时升高（P<0.05）；各组大鼠血清IL-1β含量均在造模后第1天时下降（P<0.05），后逐渐恢复，但恢复速度不一；单纯烧伤组及烧伤后多次短时光照组大鼠血清IL-6含量均在造模后第1天时升高（P<0.05），后下降，但单纯烧伤组下降幅度大于烧伤后多次短时光照组，而烧伤后单次短时光照组于造模后第3天升高后下降，烧伤后单次长时光照组呈持续升高态势（P<0.05）。单纯烧伤组及烧伤后单次长时光照组大鼠静脉血白细胞计数均于造模后第1天时升高并维持（P<0.05），其余各组白细胞计数无明显改变。烧伤后单次短时光照组、烧伤后单次长时光照组和烧伤后多次短时光照组大鼠创面愈合率均高于单纯烧伤组（P<0.05）。结论 全身LLLT可改善严重烧伤大鼠血清炎症因子TNF-α、IL-1β、IL-6的含量及静脉血白细胞计数，促进创面愈合，且不同照射模式所产生的效果不一。</br>Objective To explore the effect of systemic low-level light therapy (LLLT) on early inflammatory response of severe burn rats. Methods Fifty SD rats were randomly divided into control group, burned model group, single short-term LLLT group, single long-term LLLT group and the repeated short-term LLLT group, with 10 rats in each group. After burning the rats in the single short-term LLLT group, the single long-term LLLT group and the repeated short-term LLLT group were treated by low-intensity LED irradiation (640 nm) for 5 min once a day, 15 min once a day and 5 min three times a day, respectively.The levels of tumor necrosis factor α (TNF-α), interleukin (IL)-1β and IL-6 and the leukocyte count in caudal vein were determined at 1 day before modeling, immediately after modeling and on the 1st, 3rd, 7th and 14th day after modeling; and the wound area was measured on the 3rd, 7th and 14th day after modeling. The wound healing rate was calculated. Results Compared with the control group, the serum TNF-α levels in the burned model and single short-term LLLT groups were significantly increased on the 1st day after modeling (P<0.05), and the serum TNF-α levels in the single long-term LLLT group on the 1st day and the repeated short-term LLLT group on the 7th day were significantly increased (P<0.05); the serum IL-1β levels were significantly decreased on the 1st day after modeling in all groups (P<0.05), and then gradually recovered with the varied recovery rates; the serum IL-6 levels in the burned model and the repeated short-term LLLT groups were significantly increased on the 1st day after modeling (P<0.05), then decreased; and the decrease of the burned model group was greater than that of the repeated short-term LLLT group. While the serum IL-6 level
%K 光疗法 烧伤 全身炎症反应 肿瘤坏死因子α 白介素1β 白介素6 白细胞 创面愈合率&lt
%K /br&gt
%K phototherapy burns systemic inflammatory response tumor necrosis factor-α interleukin-1β interleukin-6 leukocytes wound healing rate
%U http://www.ajsmmu.cn/ajsmmu/ch/reader/view_abstract.aspx?file_no=20170114&flag=1