%0 Journal Article %T 抗草甘膦转基因油菜与野芥菜回交3代子1代和子2代的适合度研究 %A 张庆玲 %A 王建 %A 强胜 %A 王晓蕾 %A 闫静 %A 伏建国 %A 宋小玲 %J 南京农业大学学报 %D 2017 %R 10.7685/jnau.201609030 %X [目的]携带抗性基因回交后代的适合度是抗除草剂转基因作物的抗性基因能否成功逃逸到野生近缘种的重要依据。本文研究抗草甘膦转基因油菜与野芥菜的回交3代子1代（BC3F2）和子2代（BC3F3）在田间条件下的适合度，为转基因油菜基因漂移的生态风险评估提供有价值的参考。[方法]以野芥菜、抗草甘膦转基因油菜与野芥菜的BC3F2和BC3F3为材料，研究在低密度（每区15株）和高密度（每区30株）单种及不同种植比例（野芥菜、回交后代的混种比例分别为4:1、3:2和1:1）混种条件下，野芥菜与BC3F2和BC3F3的总适合度。[结果]单种条件下，BC3F2的总适合度均与野芥菜无显著性差异。混种条件下，种植比例为4:1时，BC3F2在2种密度下的总适合度均显著低于野芥菜；在混种比例为3:2和1:1条件下，BC3F2在低密度条件下的总适合度与野芥菜相当，而在高密度下的总适合度显著低于野芥菜。BC3F3在各种种植条件下的总适合度均与野芥菜无显著差异。[结论]BC3F2的总适合度受种植密度和比例的影响，在低密度3:2和1:1混种比例下的总适合度与野芥菜相当；BC3F3的总适合度不受种植密度和比例的影响。抗草甘膦转基因油菜与野芥菜的BC3F2和BC3F3都具有在野外生存定植的可能性，且BC3F3定植的可能性较BC3F2更大。因此在防范转基因油菜基因逃逸的策略上，除防范初始杂交发生外，也应该防范回交后代的产生。</br>[Objectives]The risk of gene flow from genetically modified (GM)crops to their wild relatives was determined by the fitness of the F1 hybrids and subsequent generations. We analyzed composite fitness of BC3F2 and BC3F3 between glyphosate-resistant transgenic oilseed rape (Brassica napus)and wild B. juncea in field. The findings are valuable for assessing ecological impacts caused by transgene escape from glyphosate-resistant transgenic oilseed rape to wild relatives. [Methods]The composite fitness of BC3F2 and BC3F3 between glyphosate-resistant transgenic oilseed rape and wild B. juncea and wild B. juncea were analyzed in field at low (15 plants per plot)and high density (30 plants per plot)in pure plots and different mixed plots (the ratio of wild B. juncea:backcross generation were 4:1,3:2 and 1:1). [Results]The composite fitness of BC3F2 was similar to that of wild B. juncea in pure plots. In mixed plots,compared with their respective wild B. juncea,BC3F2 had significant lower composite fitness under the 4:1 proportion at both low and high density. Under the 3:2 and 1:1 proportion,BC3F2 displayed similar composite fitness at low density and significant lower ones at high density,respectively. The composite fitness of BC3F3 was similar to that of wild B. juncea at different densities and planting proportions. [Conclusions]It implied that BC3F2 exhibited similar composite fitness to wild B. juncea under 3:2 and 1:1 plant proportions at low density although the composite fitness of BC3F2 was affected by planting densities and proportion. However,BC3F3 always had similar fitness compared with wild B. juncea. Thus BC3F2 and BC3F3 between glyphosate-resistant transgenic oilseed rape and wild B. juncea have the potential possibility to establish populations in field. Moreover,BC3F3 displayed larger possibility than BC3F2 did. Therefore,initial hybridization between transgenic oilseed rape and wild B. juncea,as well as backcross between wild B. juncea and F1 %K 野芥菜 %K 抗草甘膦转基因油菜 %K 回交后代 %K 适合度< %K /br> %K wild Brassica juncea %K glyphosate-resistant transgenic oilseed rape(Brassica napus) %K backcross progenies %K fitness %U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201703009