%0 Journal Article %T 脱氧血腐镰刀菌烯醇对小鼠骨髓源性树突状细胞表型和吞噬功能的影响 %A 夏兵兵 %A 汪袁 %A 樊彦红 %A 李银环 %A 王晴晴 %A 孙卫东 %A 张海彬 %A 何成华 %J 南京农业大学学报 %D 2017 %R 10.7685/jnau.201601003 %X [目的]本试验旨在研究脱氧血腐镰刀菌烯醇（deoxynivalenol，DON）对脂多糖（lipopolysaccharide，LPS）刺激的小鼠骨髓源性成熟树突状细胞（mature dendritic cell，mDC）的表型和吞噬功能的影响。[方法]体外培养小鼠骨髓细胞诱导分化成未成熟的树突状细胞，分别用不同质量浓度的DON（0、125、250、500 ng？mL-1）处理DC 6 h后，再加入LPS刺激18 h。以仅用LPS处理的为阳性对照组。用流式细胞仪检测DC表型（CD80、CD86和MHC-Ⅱ）变化及吞噬右旋葡聚糖的能力；RT-PCR检测细胞中细胞因子（IL-6、IL-10、IL-12和TNF-α）mRNA的相对表达。[结果]LPS刺激引起mDC表面的CD80、CD86和MHC-Ⅱ升高（P<0.05），吞噬右旋葡聚糖能力下降，IL-6、IL-10、IL-12和TNF-α的mRNA相对表达水平提高（P<0.05）；低浓度和中浓度DON（125和250 ng？mL-1）对DC的表型和免疫功能没有影响（P>0.05），高浓度DON（500 ng？mL-1）引起mDC共刺激分子（CD80、CD86和MHC-Ⅱ）表达水平降低（P<0.05），同时细胞因子的mRNA表达降低（P<0.05），但吞噬能力提高12.2%。[结论]高浓度DON对LPS刺激的mDC表型和细胞因子表达具有一定的抑制作用。</br>[Objectives] In this study,the effects of Deoxynivalenol(DON) on phenotype and phagocytosis of murine bone marrow-derived dendritic cells induced by lipopolysaccharide(LPS) were investigated. [Methods] The murine bone marrow cells from C57BL/6 mouse were cultured and induced to immature dendritic cells(iDC). iDCs were exposed to DON(0,125,250 and 500 ng？mL-1) for 6 h before adding LPS(1 μg？mL-1),and then further incubated for 18 h. Cells only incubated with LPS were used as positive controls. The phenotypic changes(CD80,CD86 and MHC-Ⅱ) and phagocytic ability of FITC-dextran in DC were analyzed by Flow cytometry. Relative expressions of cytokines(IL-6,IL-10,IL-12 and TNF-α) were assayed by real-time PCR(RT-PCR). [Results] The phenotypic expressions of CD80,CD86,and major histocompatibility complex(MHC-Ⅱ) were up-regulated on LPS-stimulated immature dendritic cells(P<0.05). However,the phagocytic ability of FITC-dextran was reduced. The gene expressions of cytokines(IL-6,IL-10,IL-12 and TNF-α) were increased(P<0.05). Meanwhile,we found that the phenotypic and immune function of LPS-induced DCs at the level of 125 and 250 ng？mL-1 DON were not changed(P>0.05). The expressions of phenotypic markers(CD80,CD86,MHC-Ⅱ) were down-regulated at the level of 500 ng？mL-1DON(P<0.05). At the same time,gene expressions of IL-6,IL-10,IL-12,and TNF-α were reduced(P<0.05). However,the phagocytic ability of FITC-dextran was elevated by 12.2%. [Conclusions] Our data indicated that DON would play a negative role in the maturation of DCs induced by LPS. This research provided the experimental basis for the immune suppression induced by DON %K 脂多糖 %K 表型 %K 吞噬功能< %K /br> %K deoxynivalenol %K denritic cells %K lipopolysaccharide %K phenotype %K phagocytesis %U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201702018