%0 Journal Article %T 从亲和性及F1的适合度评价抗除草剂转基因油菜向不同种群野芥菜的基因漂移风险 %A 王建 %A 闫静 %A 张庆玲 %A 郑爱琴 %A 强胜 %A 刘琳莉 %A 伏建国 %A 宋小玲 %J 南京农业大学学报 %D 2016 %R 10.7685/jnau.201601050 %X [目的] 了解抗除草剂转基因油菜(Brassica napus)向不同种群野芥菜(wild B.juncea)漂移的可能性,为抗除草剂转基因油菜的安全性评估提供依据。[方法] 以抗草甘膦和抗草丁膦转基因油菜为父本,17个种群野芥菜为母本,研究人工授粉条件下的结实能力和抗性基因的传递频率及F1在温室(套袋自交)和田间(开放授粉)的适合度;统计F1后代的出苗率及抗性基因的传递频率。[结果] 供试的野芥菜授2种转基因油菜花粉后每角饱粒数大于8粒;获得的F1植株对相应除草剂都具有抗性;温室和田间种植后营养生长良好;温室条件下自交不结实;田间种植后单株有效角果数、角果长及每角饱粒数均显著低于各自的亲本野芥菜,单株有效角果35.42~147.00个,每角饱粒数0.19~0.78;绝大多数F1的总适合度和各自的亲本野芥菜无显著差异。各F1后代种子都有20%以上正常出苗,且有8.33%~77.78%的后代表达对相应除草剂的抗性。[结论] 2种抗除草剂转基因油菜的抗性基因具有相似的向各种群野芥菜漂移的可能性。在我国释放转基因油菜务必防范向野芥菜的基因漂移。</br>[Objectives] In order to assess the potential gene flow from herbicide-resistant transgenic oilseed rape to different wild Brassica juncea.[Methods] The fully seeds per silique was counted after hand-pollination using 17 populations of wild Brassica juncea as maternal parent and glyphosate-resistant or glufosinate-resistant transgenic oilseed rape as paternal parent. The transgene transmitted frequencies to F1 and fitness of F1 were evaluated. The emergence rate and surviving rate after herbicide selection of the F1 progeny obtained in the field were tested.[Results] Hand-pollination produced at least 8 full seeds per silique. All F1 showed resistance to glyphosate or glufosinate. F1 plants grew well both in the greenhouse and field,produced no seeds under self-pollination in greenhouse,while,produced 35.42-147.00 siliques per plant and 0.19-0.78 full seeds per silique in the field. Finally,the composite fitness of majority F1 was similar with that of their respective female parent. Furthermore,more than 20% seeds of F1 progeny germinated and approximately 8.33%-77.78% seedlings of F1 progenies demonstrated resistance to the herbicide.[Conclusions] It is possible and similar of gene flow from transgenic glyphosate- or glufosinate-resistant oilseed rape to different wild B.juncea. Such ecological influence should not be ignored %K 野芥菜 %K 转基因油菜 %K 亲和性 %K F1 %K 适合度< %K /br> %K wild B.juncea %K transgenice oilseed rape %K compatibility %K F1 %K fitness %U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201604006