%0 Journal Article %T 光氮互作对拟南芥col-0和CS3721512突变体碳、氮代谢的影响 %A 李？t %A 杨顺瑛 %A 郝东利 %A 苏彦华 %J 南京农业大学学报 %D 2018 %R 10.7685/jnau.201801049 %X [目的]本文旨在了解光氮互作对拟南芥碳、氮代谢的影响。[方法]以野生型拟南芥col-0和谷氨酰胺合成酶（glutamine synthetase，GS1；1）的T-DNA插入突变体CS3721512为材料，采用水培方法，设置2种光照强度（正常和50%遮阳网遮阳处理）和2种NH4+水平（2和20 mmol？L-1 NH4Cl），测定拟南芥鲜质量，分析叶绿素a含量、叶绿素b含量、叶绿素总量、可溶性糖含量、可溶性蛋白含量、总N量和谷氨酰胺合成酶（GS）与谷氨酸脱氢酶（GDH）活性等生理指标。[结果]正常光照下，增加NH4+浓度，植株的鲜质量减少，而叶绿素a与叶绿素b含量增加，可溶性蛋白和可溶性糖含量增加，游离铵含量与总N量增加，GS和GDH活性升高；与col-0相比，CS3721512对高NH4+更为敏感，铵同化能力受到抑制，植株的长势更弱。弱光胁迫下，拟南芥的鲜质量下降，叶绿素a含量和叶绿素总量降低，可溶性糖和可溶性蛋白含量减少，游离铵含量与总N量降低，GS和GDH活性降低。弱光胁迫下增加NH4+浓度，可以增加拟南芥鲜质量、叶绿素a含量、游离铵含量和植株总N量，提高叶片中GS和GDH活性。[结论]光、氮及其互作对拟南芥的碳、氮代谢有影响，弱光胁迫条件下增加NH4+浓度可以增加叶片叶绿素含量，在一定程度上缓解弱光对植株的生长胁迫。与col-0相比，弱光下增加NH4+浓度，对CS3721512的生长更为有利。</br>[Objectives]The objectives of this study were to reveal the effects of light and nitrogen interaction on carbon metabolism and nitrogen metabolism in Arabidopsis thaliana.[Methods]A water experiment was conducted using Arabidopsis thaliana col-0 and mutant CS3721512(glutamine synthetase,GS1;1 T-DNA mutant) with two nitrogen levels(2 and 20 mmol？L-1 NH4Cl) and two light treatments(No shading,50% shading)to study the effects of light,nitrogen and their interaction on fresh weight,chlorophyll a,chlorophyll b,total chlorophyll content,soluble sugar content,soluble protein content,ammonium content,total N and the activities of glutamine synthetase(GS),glutamate dehydrogenase(GDH).[Results]Under normal light,with the increasing of NH4+ the fresh weight reduced,but the chlorophyll a and chlorophyll b content increased. The soluble sugar content and soluble protein content also increased. The ammonium content and the total N of increased. The GS activity and GDH activity also increased. Compared with col-0,CS3721512 was insensitive to high NH4+. The ammonium assimilation capacity of CS3721512 was inhibited,and the CS3721512 growth was weaker. Under shading,fresh weight,chlorophyll a and chlorophyll b content,content of soluble sugar,content of soluble protein,ammonium content,total N,GS activity and GDH activity reduced. With the increasing of NH4+,the fresh weight,the chlorophyll a content,the ammonium content,the total N content,GS activity and GDH activity were significantly increased.[Conclusions]The carbon and nitrogen metabolism of Arabidopsis thaliana was affected by the light,nitrogen and its interaction. So,nitrogen can partially alleviate the effect of damage by weak light stress and improve photosynthetic traits,partially alleviating the stress of plants under weak light. Compared with col-0,it is good for CS3721512,growth by weak light stress with high nitrogen %K 拟南芥 %K 光 %K 氮 %K 互作 %K 碳代谢 %K 氮代谢< %K /br> %K Arabidopsis thaliana %K light %K nitrogen %K interaction %K carbon metabolism %K nitrogen metabolism %U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201805015