%0 Journal Article
%T EGCG对铜绿微囊藻的抑制效应及机制研究
%A 吴颖川
%A 邹琳
%A 汪瑾
%A 张小倩
%A 卢亚萍
%J 南京农业大学学报
%D 2016
%R 10.7685/jnau.201506007
%X [目的]本文旨在研究EGCG对铜绿微囊藻的抑制效应,初步揭示其抑藻机制,为高效环保型抑藻剂的开发利用奠定基础。[方法]表没食子儿茶素没食子酸酯(EGCG)是茶叶中组分最高的一种儿茶素,对铜绿微囊藻具有高效而持续的抑制作用。分别用8、12和16 mg？L-1的EGCG处理对数生长期的铜绿微囊藻培养液,测定藻细胞的生长状态和生理生化活性。[结果]EGCG处理后藻细胞生长受到不同程度的抑制;光合作用效率下降;细胞内活性氧水平升高,抗坏血酸能缓解氧化胁迫。电镜观测结果显示:EGCG处理导致藻细胞内类囊体结构遭到破坏,细胞皱缩变形;藻细胞培养液中的藻毒素含量在处理前后没有显著变化,细胞内藻毒素含量显著下降。[结论]EGCG对铜绿微囊藻的生长和繁殖造成很强的抑制作用,是一种很有潜力的抑藻剂。</br>[Objectives]The aim of the study is to explore the inhibitory effect of epigalloctechin-3-gallate(EGCG)on Microcystis aeruginosa and reveal the inhibitory mechanism,which can provide the foundation for development and utilizalation of the efficient and eco-friendly algicide. [Methods]Epigallocatechin gallate is the main catechin in tea,which has continuously and efficiently inhibitory effect on M.aeruginosa. The culture of M.aeruginosa in logarithmic phase was treated with 8,12 and 16 mg？L-1 EGCG. And then the biomass,physiological and biochemical activities of algal cells were determined. [Results]The growth of algal cells was inhibited by the three concentrations of EGCG at different degree and the photosynthesis efficiency declined. Moreover,active oxygen in the cells increased,and ascorbic acid could relieve the oxidative stress. Transmission electron microscopy observations showed that the structure of thylakoid was destroyed and the cell was shrunk and deformed. In addition,algal toxins in the cell culture solution had no significant change between treatment and control,but algal toxins inside the cells were significantly decreased. [Conclusions]EGCG has a strong inhibitory effect on M.aeruginosa,which is considered as a promising algicide
%K 铜绿微囊藻
%K EGCG
%K 抑藻
%K 机制&lt
%K /br&gt
%K Microcystis aeruginosa
%K EGCG
%K algal inhibition
%K mechanism
%U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201601011