%0 Journal Article
%T 水稻黄叶突变体yl的遗传分析与基因定位
%A 蒋悦
%A 孙娟
%A 韩思迪
%A 范磊
%A 许凯文
%A 江玲
%A 王春明
%J 南京农业大学学报
%D 2016
%R 10.7685/jnau.201604004
%X [目的]水稻叶片作为重要的光合器官，是作物产量的形成基础。叶色突变体不仅可以作为育种标记，还是研究叶绿体发育和培育高光效水稻品种的先决条件之一。[方法]从籼稻品种‘9311’的60Co-γ射线辐射诱变突变体库中筛选获得了1个水稻黄叶突变体yl。利用yl/‘宁粳4号’杂交的F2群体进行基因定位。[结果]与野生型相比，yl突变体的幼叶表现出明显的黄化，叶绿素a、叶绿素b、类胡萝卜素的含量下降。突变体叶绿体中类囊体片层结构排列松散。与野生型相比，yl突变体的每穗粒数、结实率和千粒质量下降。该性状受1对隐性核基因控制。该基因初步定位于第9染色体长臂上，进一步开发分子标记把定位区间缩小在14.5 kb内，该区域包含3个候选基因。经测序比对分析发现，yl突变体的第3个ORF的第5外显子发生碱基突变（2131CTA→G），从而造成蛋白第233位氨基酸发生改变并导致翻译提前终止，形成了1个包含原有232个氨基酸和16个新形成氨基酸残基的蛋白，该基因与已报道的水稻黄叶基因YLC1是等位基因。[结论]本研究明确了YLC1参与叶绿素的合成，为叶绿体色素合成和叶绿体发育的研究提供了基础。</br>[Objectives] Leaf,a crucial organ for photosyhthesis,provides the basis of crop yield formation. The leaf color mutants can be used not only as a trait marker in breeding,but also as one of the prerequisites in chloroplast development and cultivation of new germplasms with higher photosynthetic efficiency. [Methods] A yellow leaf mutant,named as yl,was isolated from the progeny of 60Co-γ-rays treated indica cultivar‘9311’.The gene was isolated using an F2 mapping population derived from the cross of the yl mutant and‘Ningjing 4’. [Results] The yl mutant exhibited a stable yellow leaf phenotype during the seedling stage. As compared with the wild-type,the content of chlorophyll a,chlorophyll b and carotenoid reduced in yl leaves.The yl mutant showed less grain number per panicle,less seed setting rate and fewer 1 000-grain weight. The yellow leaf phenotype of the yl mutant was caused by a single recessive gene located in a 14.5 kb region on chromosome 9 containing three ORFs. The yl gene had nucleotide changes(2130CTA→G) in the fifth exon of the 3rd ORF,which led to frame shift and early termination in the process of translating protein. The gene was a novel allele of the YLC1 gene. [Conclusions] We demonstrated that the YLC1 gene is associated with the chlorophyll synthesis,providing a basis for further study on chloroplast pigment synthesis and chloroplast development
%K 水稻
%K 黄叶突变体
%K 基因定位
%K 候选基因分析
%K 叶绿体发育&lt
%K /br&gt
%K rice(Oryza sativa L.)
%K yellow leaf mutant
%K gene mapping
%K candidate gene analysis
%K chloroplast development
%U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201606001