%0 Journal Article
%T 蕾薹期干旱对油菜生长和抗氧化特性的影响及三唑酮的缓解效应
%A 钟飞燕
%A 张曼
%A 戴蓉
%A 江海东
%A 周琴
%J 南京农业大学学报
%D 2016
%R 10.7685/jnau.201603006
%X [目的] 干旱是限制作物生产的主要限制因子，如何采取有效措施缓解干旱胁迫具有重要意义。[方法] 以‘南农油4号’为材料，通过盆栽试验，于蕾薹期进行干旱处理，试验设置3个处理：正常对照、干旱处理和旱后三唑酮（TDM）处理，研究了叶面喷施TDM对蕾薹期干旱胁迫下油菜生长、抗氧化特性及产量的影响。[结果] 随着干旱时间的延长，油菜生长缓慢，叶片叶绿素含量和相对含水量下降，气孔关闭。叶片渗透调节能力下降，膜脂过氧化程度加重，抗氧化性能减弱，产量显著下降。TDM能够有效增强干旱胁迫下油菜持水能力，减缓叶绿素含量的下降，促进气孔开放，使叶片上张开的气孔数目显著增多。干旱胁迫后喷施TDM处理显著提高干旱胁迫下油菜叶片抗氧化酶（SOD、POD、CAT、APX、GPX等）的活性、非酶抗氧化剂（AsA、GSH）的含量和叶片渗透调节物质（可溶性蛋白、脯氨酸、可溶性糖、蔗糖）的含量，抑制了H2O2含量的增加，降低O2？的产生速率；明显提高了油菜主花序角果数、千粒质量和单株产量。[结论] 干旱胁迫打破油菜渗透调节平衡，降低其抗氧化能力，抑制油菜生长，降低油菜籽产量；TDM通过提高渗透调节能力与抗氧化性能，增强油菜抗旱性，一定程度上缓解了产量的下降。</br>[Objectives] Drought is one of the most important abiotic stress factors limiting agricultural production. The objectives of this study were to determine the alleviative effects of triadimefon(TDM) on the growth and antioxidant in oilrape under drought at bolting-stage. [Methods] A pot experiment was carried out to study the alleviated effects of foliar spraying TDM on growth,antioxidant ability and yield of oilrape under drought at bolting-stage with oilrape variety(Brassica napus ‘Nannongyou 4’). These treatments were set as control(CK),drougt stress treatment(D),and drougt stress with TDM treatment(DT). [Results] With the prolonging of the drought time,oilrape growth rate was reduced by drought relative to the control(CK).Chlorophyll content and the relative water content in leaf decreased with drought. Drought induced stomatal closure. The osmotic adjustment ability and antioxidant ability declined,while leaf membrane liquid peroxidation ascended under drought. As a result,the yield of oilrape decreased seriously. DT(TDM treated in drought of five days) could effectively enhance the water holding capacity of oilrape under drought,alleviated the reduction in chlorophyll content,and promote the opening of stomata which was showed as increasing the number of open stoma significantly. The activity of antioxidant enzymes(SOD,POD,CAT,APX,GPX,etc.) and content of non-enzymatic antioxidants(AsA,GSH) and osmotic regulating substance significantly increased and the accumulation of H2O2 and O2？ was inhibited in DT leaves. As a result,pod number in main inflorescence,1 000-grain weight and yield per plant in DT were significantly improved as compared with those in D(drougt stress treatment). [Conclusions] Drought broke osmotic adjustment balance and decreased antioxidant activity in oilrape. It retarded the growth of the plant,and led to a serious decline in yield. TDM under drought can improve the drought resistance of oilrape by improving the antioxidant properties
%K 油菜
%K 蕾薹期
%K 干旱
%K 三唑酮
%K 抗氧化特性
%K 产量&lt
%K /br&gt
%K oilrape
%K bolting-stage
%K drought
%K triadimefon (TDM)
%K antioxidant properties
%K yield
%U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201605004