%0 Journal Article
%T 羟丙基-β-环糊精包合穿心莲内酯及其衍生物的抑菌活性研究
%A 张丹妮
%A 黄瑶
%A 乔颖
%A 夏彩萍
%A 骆宇强
%A 陈志刚
%J 南京农业大学学报
%D 2016
%R 10.7685/jnau.201509035
%X [目的] 本文旨在提高穿心莲内酯(AND)及其乙酰化衍生物(AcAND)的水溶性并探究其抑菌效果及机制。[方法] 用羟丙基-β-环糊精(HP-β-CD)对AND和AcAND进行包合,以食品中常见的4种致病菌(大肠杆菌、单增李斯特氏菌、沙门氏菌、金黄色葡萄球菌)为试验菌株,采用菌落计数的方法研究上述包合物对菌株生长的影响。通过测定抑菌圈、最小抑菌浓度(MIC)、最小杀菌浓度(MBC)检测其抑菌效果,并通过测定菌液上清液中β-半乳糖苷酶(β-GAL)、碱性磷酸酶(AKP)及总蛋白水平以研究包合物的抑菌机制。[结果] AND/AcAND-HP-β-CD的最佳紫外检测波长为224 nm,且HP-β-CD显著提高了AND和AcAND的水溶性。抑菌试验表明,AND-HP-β-CD无明显抑菌活性,而AcAND-HP-β-CD对革兰氏阳性菌和革兰氏阴性菌均显示出了很好的抑菌活性,其中对大肠杆菌的抑菌效果最佳。初步抑菌机制试验表明,AcAND-HP-β-CD可有效破坏菌体细胞壁和细胞膜的完整性,造成β-GAL、AKP以及胞内蛋白的大量外泄。[结论] HP-β-CD可显著改善AND和 AcAND的水溶性,酶法修饰可提高物质的抑菌活性,此研究为穿心莲内酯及其衍生物在食品及医药行业中的应用提供了试验依据。</br>[Objectives] The study aims to improve the aqueous solubility of andrographolide(AND)and 14-acetylandrographolide(AcAND)and explore their antibacterial effect and mechanism. [Methods] Hydroxypropyl-β-cyclodextrin(HP-β-CD)was used to form inclusion complexes with AND and AcAND. Four kinds of foodborne pathogenic bacteria strains(Escherichia coli,Staphylococcus aureus,Salmonella enterica,Listeria monocytogenes)were used to test the effect of AND/AcAND-HP-β-CD on their growth by counting colony and measuring the inhibition zone,the minimum inhibitory concentration(MIC),the minimum bactericidal concentration(MBC),β-galactosidase(β-GAL)content,alkaline phosphatase(AKP)content and total protein concentration. [Results] The results showed that optimum detection wavelength of AND/AcAND-HP-β-CD was 224 nm and HP-β-CD could significantly improve the aqueous solubility of AND and AcAND. Antibacterial research demonstrated that AND-HP-β-CD did not show antibacterial activity,while AcAND-HP-β-CD showed good antibacterial activity especially on E.coli. Preliminary antibacterial mechanism showed that AcAND-HP-β-CD undermined the integrity of bacterial cell walls and outer membranes,resulting in the leak of β-galactosidase(β-GAL)content,alkaline phosphatase(AKP)and intracellular proteins. [Conclusions] HP-β-CD can improve the aqueous solubility of AND and AcAND. And enzymatic modification is the way to increase the antibacterial activity. Conclusively,this study can provide an experimental foundation for andrographolide and its derivatives used in the food and pharmaceutical industries
%K 羟丙基-β-环糊精
%K 穿心莲内酯
%K 乙酰化穿心莲内酯
%K 抑菌活性&lt
%K /br&gt
%K hydroxypropyl-β-cyclodextrin
%K andrographolide
%K 14-acetylandrographolide
%K antibacterial activity
%U http://nauxb.njau.edu.cn/oa/darticle.aspx?type=view&id=201602020