%0 Journal Article
%T 大叶桉炭疽病病原菌的鉴定及室内药剂筛选
%A 曾学英
%J 林业工程学报
%D 2015
%R 10.13360/j.issn.1000-8101.2015.04.030
%X 2014年笔者首次在四川攀枝花大叶桉种植区内发现疑似大叶桉炭疽病病害，通过对发病植株病健交界处叶片采样、分离、单孢培养及致病性测定，得到1株具有代表性的菌株Z-D-07，结合形态学以及基于rDNA？？ITS序列的系统发育对该菌株的种类进行鉴定。结果表明，该菌株形态学特性与博宁刺盘孢Colletotrichum boninense一致；利用真菌通用引物ITS1和ITS4进行rDNA的扩增，对扩增产物进行回收、克隆、测序，得到597 bp的基因片段，通过登陆NCBI进行Blast比对，注册登录号为KJ619456，并建立同源性较高的Colletotrichum属菌株的系统发育树，确定菌株Z-D-07为炭疽菌属胶胞炭疽菌Colletotrichum boninense。分别选用6种常见农药对大叶桉炭疽病菌进行室内药剂防治试验，发现70%代森锰森锰锌防治效果最好，80%炭疽福美次之。</br>We first discovered a suspected disease named anthrax Eucalyptus robusta planting regions in Panzhihua, Sichuan, in 2014. We obtained the representative strain Z-D-07 with the methods of sampling, separation, single spore culture, as well as pathogenicity tests, and we also identified the species of Z-D-07 based on morphological and developmental systems rDNA？？ITS sequences. The results showed that the strain Z-D-07 in morphological characteristics were consis？？tent with Colletotrichum boninense. By using the fungal universal primers ITS1 and ITS4, we amplified the internal transcribed spacer rDNA sequences of the strain Z-D-07, then after recyclying, cloning and sequencing the amplification products, a length of 597 bp gene fragments was obtained. The results of blast in Genebank, the analysis of fungal rDNA systemic development and phylogenetic tree showed that the relation between this fragment and the fungi of Colletotrichum boninense was closest, so morphological and molecular identification confirmed this fungal pathogen strain Z-D-07 as Colletotrichum boninense. Six kinds of common pesticides were used to study the control effects on Eucalyptus anthrax in the test, the results showed that 70% mancozeb presentd the optimum control efficiencies, and 80%thiram took second place
%K 大叶桉
%K 炭疽病
%K 盘长孢状刺盘孢
%K 系统发育树
%K 药剂防治&lt
%K /br&gt
%K Eucalyptus robusta Smith
%K anthrax
%K Colletotrichum boninense
%K phylogenetic tree
%K termiticides
%U http://lkkf.njfu.edu.cn//oa/darticle.aspx?type=view&id=201504031