%0 Journal Article
%T 茶皂素对稀酸预处理锯木屑酶水解的影响
%A 杨博
%A 赖晨欢
%A 林子贺
%A 谢佳笑
%A 宋向阳
%J 林业工程学报
%D 2018
%R 10.13360/j.issn.2096-1359.2018.06.013
%X 高效纤维素酶水解是木质纤维原料生物炼制的关键。通过添加表面活性剂减少纤维素酶在物料上的非特异性吸附是提高酶水解效率的有效方法之一。然而,目前有关天然表面活性剂在木质纤维素酶水解中的应用鲜有报道。该研究介绍了一种从油茶饼废弃物中提取的天然表面活性剂――茶皂素在木质纤维素酶水解中的应用,并探究其对预处理物料酶水解的促进机制。结果表明,在稀酸预处理锯木屑酶水解中,茶皂素最适添加量为0.075 g/g。当纤维素酶用量为20～80 U/g时,添加0.075 g/g的茶皂素使得稀酸预处理锯木屑72 h葡萄糖得率提高了10.3%～32.5%。同时,添加茶皂素可显著提高72 h酶水解上清液中的残余纤维素酶活力,其中内切葡聚糖酶和滤纸酶活分别提高了6.6和3.0倍。由此推测,天然表面活性剂茶皂素可减少纤维素酶在物料上的非特异性吸附,从而提高预处理物料酶水解效率。此外,添加茶皂素相对含量为0.075 g/g的油茶饼茶皂素粗提液时,稀酸预处理锯木屑葡萄糖得率同样提高了33.3%。综上所述,茶皂素作为一种天然表面活性剂用于木质纤维生物炼制具有一定的应用前景,为油茶饼废弃物开拓了新的利用途径。</br>Cellulase hydrolysis of cellulose is a dominating process in lignocellulose biorefinery. To achieve the highly efficient enzymatic hydrolysis, it is necessary to conduct the pretreatment on lignocellulosic materials prior to the enzymatic hydrolysis. The pretreatment on lignocelluloses can destroy the recalcitrant structure of the lignocellulosic matrix, and thus increase the cellulose accessibility. However, the residual lignin after pretreatment still hindered the enzymatic hydrolysis of lignocellulosic materials by physically blocking and non-productively binding cellulases. The addition of surfactant is considered as one of the effective methods to improve the enzymatic hydrolysis, due to its role of reducing the non-productive adsorption of cellulase. Tea saponin, a kind of natural surfactant and easily obtained from oil tea cake waste, was applied in the enzymatic hydrolysis of dilute acid pretreated wood sawdust in this paper. The results showed that the addition of tea saponin could enhance the efficiency of enzymatic hydrolysis of dilute acid pretreated wood sawdust. The optimal loading of tea saponin was 0.075 g/g(based on dry weight of cellulose). Within the cellulase dosage range of 20-80 U/g cellulose, the addition of tea saponin increased the 72 h glucose yield of dilute acid pretreated wood sawdust by 10.3%-32.5%. Meanwhile, the addition of tea saponin obviously increased the residual activities of cellulase in the supernatant of 72 h hydrolysis. The endo-glucanase activity and filter paper activity respectively increased 6.6 and 3.0 fold. This indicates that the addition of tea saponin can improve the enzymatic hydrolysis of dilute acid pretreated wood sawdust by reducing the non-productive adsorption of enzyme. Moreover, the addition of the crude tea saponin solution(relative loading of 0.075 g/g based on dry weight of cellulose)from oil tea cake increased the glucose yield of enzymatic hydrolysis by 33.3%. It suggested that tea saponin could be used as a natural surfactant to promote the enzymatic hydrolysis of lignocellulosic materials
%K 茶皂素
%K 表面活性剂
%K 纤维素酶水解
%K 稀酸预处理
%K 非特异性吸附&lt
%K /br&gt
%K tea saponin
%K surfactant
%K cellulase hydrolysis
%K dilute acid pretreatment
%K non-productive adsorption
%U http://lkkf.njfu.edu.cn//oa/darticle.aspx?type=view&id=201806013