%0 Journal Article
%T 青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究
%A 徐静珠
%A 吴彩娥
%A 应瑞峰
%A 李婷婷
%A 范龚健
%A 方升佐
%J 南京林业大学学报(自然科学版)
%D 2017
%R 10.3969/j.issn.1000-2006.201610064
%X 【目的】研究青钱柳叶多糖的体外降血糖及抗氧化活性。【方法】以对α-葡萄糖苷酶的抑制作用表示青钱柳叶多糖体外降血糖活性,以总抗氧化能力、清除DPPH自由基、羟基自由基能力和Fe2+螯合能力评价其体外抗氧化活性。【结果】青钱柳叶多糖各组分对α-葡萄糖苷酶均有一定的抑制作用,且呈现一定的剂量-效应关系。不同醇沉多糖组分中,50%醇沉组分对α-葡萄糖苷酶的抑制作用最强; 青钱柳叶多糖各组分均具有一定的抗氧化活性,不同醇沉组分清除DPPH自由基能力以及总抗氧化能力由强到弱依次为50% 组分、60% 组分、70%组分、80%组分,而清除羟基自由基能力以及对Fe2+螯合能力的强弱正好相反。【结论】青钱柳叶中含有的多糖组分具有良好的体外降血糖和抗氧化活性,可进一步加工利用。</br>【Objective】In order to evaluate the hypoglycemic and antioxidant activity of polysaccharides from Cyclocarya paliurus leaves in vitro. The activity of refined polysaccharides and precipitation fractionations of purified C. paliurus polysaccharide obtained from different concentrations of ethanol were compared in this study.【Method】The activity of inhibiting α-glucosidase was used to evaluate the hypoglycemic effect in vitro. The ability of total antioxidant, scavenging DPPH and(？OH)radicals and chelating Fe2+were used to evaluate the antioxidant capacity.【Result】The results showed that refined polysaccharides of C. paliurus and different precipitation fractionations all showed a high inhibition against α-glucosidase, and showed a dose-effect relationship. With the ethanol concentration increasing, the fractions obtained showed a higher inhibition activity. Refined polysaccharides of C. paliurus and different precipitation fractionations all showed a good antioxidant activity. Among different precipitation fractionations, the abilities of scavenging DPPH and total antioxidant were increased with the ethanol concentration increasing. In contrast, the abilities of scavenging(？OH)radicals and chelating Fe2+ were decreased.【Conclusion】Different C. paliurus polysaccharides have good in vitro hypoglycemic and antioxidant activity, which can be used for further manufacture and expoliting
%K 青钱柳
%K 多糖
%K 降血糖
%K 抗氧化&lt
%K /br&gt
%K Cyclocarya paliurus
%K polysaccharide
%K hypoglycemic effect
%K antioxidant activity
%U http://nldxb.njfu.edu.cn/oa/darticle.aspx?type=view&id=201704002